SlCBL1基因调控番茄果实成熟发育的分子机理

Molecular Mechanism of SlCBL1 Regulation during Tomato Fruit Development

以番茄（Solanum lycopersicum L.）品种‘Micro Tom’为试材,从其果实中克隆得到番茄类钙调磷酸酶B基因（Tomato Calcineurin B-Like gene,SlCBL1）,构建其带有报告基因的e-GFP植物表达载体,分析番茄果实中SlCBL1基因超表达与成熟发育进程的相互关系。结果显示：（1）与对照非转基因植株以及转空载植株相比,转SlCBL1基因番茄中SlCBL1基因过量表达,而且能够使番茄果实成熟期提前3～5d,表明SlCBL1基因可促进番茄果实成熟。（2）番茄果实成熟相关基因的表达量也受到不同程度调控,其中番茄成熟过程中的色素合成基因、乙烯路径基因以及果实成熟相关转录因子都受到强烈的调控,与对照相比表达量分别上调5～10倍。研究表明,SlCBL1基因能够促进番茄果实成熟,而且通过影响色素合成基因以及果实成熟相关转录因子来调控番茄果实成熟。

With tomato （Solanum lycopersicum L.） variety ‘Micro Tom’ as test materials, we cloned the tomato Calcineurin B Like gene （SlCBL1） from ‘Micro Tom’ fruitand constructed inplant expression vector with e GFP report gene. Then we analyzed the relationship between SlCBL1 gene over expression and tomato fruit development. The results show that： （1） compared with non transgenic plants and empty vector plant, over expression SlCBL1 can promote fruit mature and made tomato fruit mature for 3 to 5 days in advance. （2） The expression of tomato fruit development related genes also regulated in different degrees. Including tomatoes mature in the process of pigment synthesis genes, the path of ethylene and ripe related transcription factors have been strongly regulated, and the expression level raised about 5 to 10 times compared with control. Results show that SlCBL1 gene can promote the fruit development, and by influencing the pigment synthesis genes and ripe related transcription factors to control tomato fruit mature.