番茄幼苗盐胁迫响应蛋白质组分析

Proteome Analysis of Tomato Seedlings in Response to Salt Stress

采用营养液栽培,以盐敏感型番茄品种M82为试材,利用双向电泳(2-DE)研究盐胁迫处理下幼苗叶片蛋白质的表达谱,并采用基质辅助激光解析飞行时间串联质谱(MALDI-TOF/TOF-MS)技术进行差异蛋白质的分离及质谱鉴定。结果表明:(1)盐胁迫处理下,利用2-DE获得差异显著蛋白点20个,其中17个蛋白质点丰度上调表达,3个蛋白质点丰度下调表达。(2)通过质谱分析和蛋白质NCBInr数据库检索,共鉴定出19个差异蛋白,分别为果糖-二磷酸醛缩酶、S-腺苷甲硫氨酸合成酶、甘油醛-3-磷酸脱氢酶等及3个功能未知蛋白;这些鉴定出的差异蛋白质与能量代谢、光合作用、蛋白合成、氧化还原平衡等过程相关,暗示所分离鉴定的蛋白可能参与了番茄的盐胁迫响应,为进一步研究番茄抗逆机制奠定基础。

Nutrient solution culture was used to M82 tomato salt sensitive variety as experimental materials. With two dimensional electrophoresis （2 DE）, we studied the salt conditions and processing tomato seedlings under salt stress protein expression profile,and identified the differences of protein separation and mass spectrometry trough tandem time of flight mass spectrometry （MALDI TOF/TOF MS） technology. The results showed that： （1） there were 20 protein spots presenting to express differentially after the total proteins were separated by 2 DE. The 17 proteins of all presenting spots were up regulated,and 3 proteins were down regulated. （2） Among the 20 protein spots,the annotations of the 19 differential expressed proteins were known by mass spectrometry and protein database retrieval,Identified differential proteins took part in fructose bisphosphate aldolase,S adenosyl L methionine synthetase,glyceraldehyde 3 phosphate dehydrogenase,etc,and three unknown functions of protein. These identified the differences of proteins involved in energy metabolism, photosynthesis,protein synthesis,redox balance and other related process,suggested that these proteins may be involved in the salt stress response of tomato,which lay a foundation for further research the mechanism to understand plant stress resistance.