魔芋飞粉中ACE抑制肽酶法制备技术研究

Enzymolysis for Preparation of ACE Inhibitory Peptides from Konjac Fly Powder

以魔芋飞粉为原料制备血管紧张素转换酶(ACE)抑制肽,为魔芋飞粉的开发利用,生产高附加值产品开辟一条新的途径。以体外ACE抑制率为指标筛选最佳用酶并确定酶解条件。结果表明:胃蛋白酶为酶解魔芋飞粉最佳用酶,其最佳酶解条件为:底物浓度2%(净蛋白),酶用量5 000 U/g底物蛋白,温度37℃,pH 2.5,酶解时间210 min,此时酶解液的ACE抑制率为92.65%,多肽得率为12.60%。魔芋飞粉经蛋白酶酶解可制备高活性的ACE抑制肽。

Open up a new way for the development and utilization of the konjac fly powder and the high added value products, the technology for preparation of angiotensinI-converting enzyme （ACE） inhibitory peptided by hydrolyzing this resource was studied. The optimum protease and the optimum hydrolysis conditions were determined for maximizing ACE inhibitory activity in vitro of konjac fly powder hydrolysate. Results showed that pepsin was the optimum protease to hydrolyze konjac fly powder protein. The optimum conditions for pepsin-catalyzed enzymolysis were as follows： substrate concentration 2%, temperature 37 ℃, enzyme concentration 5 000 U/g （substrate）, and pH 2.5 for a hydrolysis duration of 210 min. Under the optimum conditions, the ACE inhibitory ratio of konjac peptided with a yield of 12.60% was 92.65%. In conclusion, the developed enzymolysis technology was feasible for the preparation of ACE inhibitory peptided with high biological activity from konjac fly powder.