基于果胶FTIR结合主成分分析鉴别不同品种莲藕

Identification of Different Varieties of Lotus Rhizomes Based on pectin FTIR Combined with Principal Component Analysis

对三个品种莲藕的果胶进行选择性抽提,得到水溶性组分(Water soluble fraction,WSF)、CDTA组分(Chelator soluble fraction,CSF)和Na_2CO_3组分(Na_2CO_3 soluble fraction,NSF)。通过测定三个果胶组分的傅里叶红外光谱,结合主成分分析统计方法,结果表明,CSF和NSF两个果胶组分通过主成分分析只能得到一个主成分,因此,不能有效区分三个样品,而WSF果胶在主成分空间中聚集为不同的类型,且不同样品处于相对独立的空间,因此,可以基本实现不同品种莲藕的鉴别。

Three varieties of pectin components of lotus rhizomes were extracted, including water soluble fraction （WSF）, chelator soluble fraction （CSF） and Na2CO3 soluble fraction （NSF）. Fourier transform infrared （FTIR） spectroscopy was used for three pectin components, and principal component analysis （PCA） was applied to their spectra in the region between 1 250-750 cm^-1. The results indicated that, through PCA, only one principal component in two pectin components CSF and NSF was got, therefore it was impossible to distinguish between three samples. While the WSF pectin in principal components gathered into different types and different samples was in a relatively independent space, thus three varieties of lotus rhizomes could be distinguished apparently.