无柄灵芝遗传多样性的SRAP、ITS、TEF1-α和LSU分析

Genetic diversity of Ganoderma resinaceum by SRAP, ITS, TEF1-α and LSU markers

【目的】研究来自我国不同地区的45株无柄灵芝菌株的遗传多样性。【方法】利用ITS、TEF1-α和LSU多基因分析及SRAP分子标记两种方法,对供试无柄灵芝菌株进行聚类分析和遗传多样性研究。【结果】筛选出8对SRAP引物共扩增出95条条带,其中具有多态性条带79条,平均多态性比例为82.4%,多态性信息含量(PIC)变幅在0.28-0.43,平均为0.38。ITS、TEF1-α和LSU多基因序列分析结果显示,同一地域的部分菌株聚在一起,亲缘关系较近,而地域相隔较远的部分菌株也聚在同一个进化支上,其亲缘关系也很近,这与SRAP聚类分析结果相吻合。【结论】无柄灵芝菌株遗传多样性较为丰富,其遗传相似性与地理分布存在一定的相关性,ITS、TEF1-α、LSU基因及多基因分析更适合无柄灵芝分类鉴定,而SRAP分子标记更适合于无柄灵芝遗传多样性分析。

[Objective] Genetic diversity and relationship of 45 Ganoderma resinaceum strains from different areas in China were analyzed. [Methods] We studied clustering analysis and genetic diversity of these isolates using multi-gene analysis with ITS, TEF1-α, LSU sequences and SRAP technique. [Results] 8 pairs of primers on 45 strains were selected and amplificated using PCR-SRAP system, and 95 fragments were amplified, the number of polymorphic bands was 79, the average polymorphic rate was 82.4%. The PIC （polymorphism information content） value of these markers varied from 0.28 to 0.43, averaging 0.38. The results of multi-gene analysis with ITS, TEF1-α, LSU sequences and SRAP technique showed that part of the strains from the same area were clustered with each other, their kinship was close, and some strains with large gaps about the geographical distribution were also clustered in the same phylogenetic tree branch, their genetic relationship may also be relatively close, the result above was consistent with SRAP clustering analysis. [Conclusion] The genetic diversity of 45 strains was relatively abundant, and it had some correlation between genetic similarity and geographical distribution. ITS, TEF1-α and LSU sequences analysis and multi-gene analysis were more useful for classification and identification of Ganoderma resinaceum, and SRAP technique was more suitable for the genetic diversity analysis of Ganoderma resinaceum.