施氏鲟胚胎发育期中几种微量元素含量的变化

Changes in Levels of Several Trace Elements During Embryonic Development of Amur Sturgeon Acipenser schrenckii

在水温(18±0.5)℃下,利用生物化学方法测定施氏鲟Acipenserinae schrenkii胚胎发育过程中微量元素的含量,分析胚胎发育进程与微量元素含量的关系,为稚、幼鱼及亲鱼培育的营养需求量和饲料配制提供参考。结果显示:胚胎发育过程中,Fe、Mn、Cu、Zn含量的变化规律与代谢高峰期各不同。Fe含量呈先降低-升高的变化趋势,孵出期最高(18.12mg/kg);Mn含量先降低后升高,受精卵期含量最高(1.04mg/kg);Cu含量的高峰期分别为卵黄栓期、心搏期、孵化早期和孵出期,含量分别为1.66mg/kg、1.95mg/kg、1.83mg/kg和1.93mg/kg;卵裂期Zn含量最高(10.86mg/kg);视泡期微量元素的含量最低。

In this study, the changes in levels of four trace elements including Fe, Mn, Cu and Zn were determined during embryonic development of Amur sturgeon A clpenser schrenckii using biochemical methods and the relationships between the levels of four trace elements and the embryogenesis were evaluated in order to explore the nutritional requirement of Amur sturgeon juveniles as well as provide reference with development of optimal formulated feed. The results showed that the metabolic peak of the four trace elements was different during the embryonic development. During the embryogenesis, Fe content in the embryos was increased first and then de- creased, with the peak value of 18.12 mg/kg in the hatching stage, and the Mn content was shown to be increased first and then to be decreased, with the peak value of 1.04 mg/kg in the zygotes. However, the metabolic peaks of Cu were observed at yolk plug （ 1.66 mg/kg）, cardiac activity （1.95 mg/kg）, prehatching （1.83 mg/kg） and hatching stage （1.93 mg/kg）. The dynamics of Zn content was found to be stage-specific, with the maximum value of 10.86 mg/kg at cleavage stage and the minimal at optic vesicle stage. The find- ings indicate that the requirements of Amur sturgeon postlarvae and fry for the four trace elements are varied with embryonic develop- ment stages due to the metabolic peaks of different trace elements.