大鼠不同脊柱融合模型的评价及比较

Evaluation and comparison of different spinal fusion model in rats

目的比较大鼠三种不同脊柱融合模型的优越性及融合率。方法 60只SD大鼠随机分成三组,分别行椎体间(有内固定)、后外侧路(横突间)、椎体间(无内固定)三种不同术式的脊柱融合,3周、6周后进行手法触诊、X线评价、组织学观察,对三种融合模型进行分析。结果 3周时椎体间(有内固定)融合组、横突间融合组和椎体间(无内固定)融合组均未出现融合;6周时椎体间(有内固定)融合率为40%、横突间融合率为40%、椎体间(无内固定)融合率为20%。结论椎体间(有内固定)及后外侧路(横突间)融合均有良好的植骨床,局部相对稳定,手术相对简单易行,且术后并发症少,是骨替代材料等等具有成骨性或骨诱导性的移植物或药物能否提高脊柱融合率适用的一种动物融合模型。

Objective To compare the superiority and fusion rate of three different spinal fusion models in rats. Methods Sixty SD rats were randomized into three groups, A： underwent intervertebral fusions （with internal fixation）; B： underwent posterolateral lumbar fusions （intertransverse）; C： underwent intervertebral fusions （without internal fixation）. For each group, palpation, X-ray, and histology were evaluated at 3 and 6 weeks after fusion. Results All groups did not show fusion 3 weeks after fusion. The fusion rate in A and B group were 40%. However, the fusion rate in C group was only 20% 6 weeks after fusion. Conclusion Intervertebral fusions and posterolateral lumbar fusions have good and enough bone graft bed, which are feasible and easy, therefore causing less postoperative complications. In conclusion, these operations are suitable for fusion model of rat which can enhance the rate of spinal fusion with bone substitute material.