仿刺参卵多糖的分离纯化及体外抗肿瘤活性

Purification and Antitumor Activity in vitro of Polysaccharides from Apostichopus japonicus Spawn

目的:探究仿刺参卵多糖的结构及体外抗肿瘤活性。方法:通过酶解醇沉获得仿刺参卵粗多糖(polysaccharides from Apostichopus japonicus spawn,SPS);通过色谱柱纯化后进行理化性质分析;采用噻唑蓝法研究卵多糖对He La、HGC-27细胞的体外增殖抑制作用。结果:获得3种不同多糖组分SPS-1、SPS-2、SPS-3,对干预的肿瘤细胞均有显著抑制作用。SPS-1抑制作用最强,对He La及HGC-27细胞的最高抑制率分别为98.04%、99.4%。结论:从仿刺参卵中分离出3种多糖组分,均表现出一定的抑制肿瘤细胞增殖活性。

Purpose： To investigate the structural characteristics and antitumor activity in vitro of polysaccharides from Apostichopus japonicus spawn. Methods： The polysaccharides from Apostichopus japonicus spawn（SPS） were isolated by protease hydrolysis and alcohol precipitation, and then purified by chromatography. Their physicochemical properties were analyzed. Their inhibitory effect on the growth of He La and HGC-27 cells was evaluated using methyl thiazolyl tetrazolium assay. Results： SPS-1, SPS-2 and SPS-3 were purified from SPS. The proliferation of two tumor cells was inhibited by intervention of each of the three polysaccharides. SPS-1 showed the strongest antitumor activity, with a percentage inhibition of 98.04% and 99.4% for He La and HGC-27 cells, respectively. Conclusion： Three different polysaccharides with the ability to inhibit tumor cell proliferation have been obtained from A. japonicus spawn.