益生菌Lactobacillus paracasei LC01对小鼠肠道菌群的调节作用

Evaluation of Intestinal Microbiota in Mice after Oral Administration of Lactobacillus paracasei LC01

探讨益生菌Lactobacillus paracasei LC01对小鼠肠道菌群的调节作用。将32只Balb/c小鼠随机分成4组,分别作为对照组、灌胃LC01灭活菌组、灌胃LC01活菌组和活菌定殖组。干预2周后,通过Mi Seq高通量测序手段,分析不同处理条件下小鼠肠道菌群变化。结果表明:相对于对照组和灌胃灭活菌组,灌胃LC01活菌可以显著提高小鼠肠道内Lactobacillus属、Ruminococcaceae_UCG014属、Alistpes属的相对含量。进一步通过荧光定量聚合酶链式反应和群落聚类分析发现,灌胃LC01活菌不但可以显著提高小鼠肠道内乳酸菌LC01的相对含量,同时可以促进小鼠肠道中格氏乳杆菌、罗伊氏乳杆菌和唾液乳杆菌的增殖。在活菌定殖组中,停止灌胃2周后,小鼠粪便中不能检测到菌株LC01,说明菌株LC01不能在小鼠肠道内长期定殖。以上结果表明菌株LC01对肠道小鼠菌群结构具有一定的调节作用,具有潜在的益生作用和应用价值。

To evaluate the effect of Lactobacillus paracasei LC01（LC01） on the mouse intestinal microbiota, Balb/c mice were randomized and equally divided into four groups（n = 8）. The live LC01, inactive LC01 and control groups were administered with live LC01, inactive LC01 and resuspended buffer for a 2-week period, respectively. The colonization group underwent a 2-week washout period after the administration of LC01. Mi Seq high-throughput sequencing was used to determine the intestinal bacterial composition of fecal samples collected at the end of every period. A significant increase in Lactobacillus, Ruminococcaceae_UCG014 and Alistpes was observed in the live LC01 group compared with the control and inactive LC01 groups. Furthermore, species-specific quantitative polymerase chain reaction and operational taxonomic unit classification analysis revealed that LC01 could stimulate the proliferation of Lactobacillus gasseri and Lactobacillus reuteri. After the washout period, operational taxonomic units could not be detected in the fecal samples. The results indicated a modulation effect of LC01 on the intestinal microbiota in mice, suggesting that LC01 may have potential as a probiotic.