福建省猪流行性腹泻病毒的ORF3基因序列分析

Sequence Analysis on ORF3 Gene of Porcine Epidemic Diarrhea Virus in Fujian

为探讨福建省猪流行性腹泻病毒（PEDV）ORF3基因遗传变异情况,于2014-2015年从福建省不同地区猪场扩增8株PEDV的ORF3基因,并进行序列比对及遗传进化分析。结果表明：8株分离株均为野毒株;与近年来国内及其周边国家的分离株亲缘关系较近,其核苷酸同源性及推导的氨基酸同源性最高高达100%;与经典株CV777亲缘关系较远,其核苷酸及推导的氨基酸同源性达96.0%~96.9%与92.5%~94.7%;表明近年来福建省PEDV流行株较以往的经典株已经发生了改变。

To study the ORF3 gene of porcine epidemic diarrhea virus （PEDV） in Fujian, samples were collected from regions in the province during 2014- 2015. Eight strains were amplified for sequence alignment and phylogenetic analysis. Genetic evolution analysis showed that the strains were of wild variety and close to those found domestically and in the neighboring countries in recent years with up to 100% homology on the nucleotide and deduced amino acids. But, they were distantly related to the classical strain of CV777, with a nucleotide homology of 96.0 %- 96.9 ~ and amino acid homology of 92.5 %- 94.7 %. It appeared that genetic alternations had occurred to the PEDV strain currently isolated from the samples collected in the province,