蛋白质相互作用试验技术研究进展

Research Progress of Protein-protein Interaction Technology A Research Review of the Technology of Protein-protein Interaction

大多数表型功能的形成与蛋白质和基因产生的相互作用有关。蛋白质相互作用(PPI,protein-protein interaction)是研究蛋白质功能的重要手段,近年来发展较快。该技术在预测目的蛋白功能和基因功能上发挥了重要作用。蛋白质相互作用的研究方法主要分为3类:体内试验、体外试验、模拟生物学试验。本文比较了这些试验方法各自的特点及局限性,综述了蛋白质相互作用试验技术的研究进展及其在生物学研究中的应用,并对该技术研究进行了展望。

Most phenotypic functions come from the interaction between genes and proteins. Protein-protein interaction （PPI） is an important technology that has developed rapidly in recent years, to study protein function and this technology plays a key role in predicting the function of target proteins and genes. The main research methods of PPI are divided into three categories： in vivo, in vitro and in silico. This review compares the characteristics and limitations of these methods, summarizes the progress and application of protein-protein interaction technology in biology research, and prospects this technology.