摘要
从碳氮源、酸碱度及生长温度等方面对俄罗斯木霉（Trichoderma rossicum）菌株GAU 1-X-2的生物学特性进行了研究。结果表明,俄罗斯木霉菌株GAU 1-X-2的生长温度范围为10～35℃,最适温度为20℃;菌落在pH值为5.0～12.0的培养基上能够生长,pH为7.0时,生长最快,pH值为8.0时产孢量最大;营养生长最好的碳源为甘露醇,产孢量最好的碳源为葡萄糖;营养生长最适氮源为蛋白胨,在供试的7种氮源中,培养6d未观测到该菌株产孢;完全黑暗条件有利于菌丝生长和产孢;孢子致死温度为68℃,10min。
The biological characteristics of Trichoderma rossicumstrain GAU 1-X-2were examined using different carbon and nitrogen sources,pH,temperature,and other factors.The results indicated that T.rossicum could grow under temperatures of 10-35 ℃ and pH of 5.0-12.0,with optimal conditions of 20 ℃ and pH7.0,although the highest sporulation occurred at pH 8.0.Mannitol was the optimum carbon source for hypha growth,but dextrose was the best for spore production.Peptone was the optimum nitrogen source for mycelia,but conidiophores were not observed after six days in the seven nitrogen sources.The best mycelia growth and the largest sporulation occurred with 24 hof darkness.The lethal spore temperature was 68 ℃for 10 min.
出处
《草业科学》
CAS
CSCD
北大核心
2016年第12期2391-2397,共7页
Pratacultural Science
基金
甘肃省农牧厅生物技术研究与应用开发项目(GNSW2014-16
GNSW-2013-32)
农业部公益性行业(农业)科研专项"作物根腐病综全治理技术"(201503112)
甘肃省农业科学院中青年基金项目(2014GAAS25)
关键词
俄罗斯木霉
生物学特性
菌丝生长
产孢量
Trichoderma rossicum
biological characteristics
hypha growth
sporulation
