温肾养血方对无排卵大鼠抑制素-激活素-卵泡抑素系统的影响

Effect of Wenshen Yangxue Recipe on Inhibin-Activin-Follistatin System in Anovulatory Rats

目的探讨温肾养血方对无排卵大鼠抑制素—激活素—卵泡抑素(inhibin-activin-follistatin,INH-ACT-FS)系统和性腺激素水平的影响。方法 76只9日龄大鼠于颈背部皮下一次性注射丙酸睾丸酮1.25mg/只(0.05mL/只)制备无排卵大鼠模型。将58只造模成功大鼠按随机数字表法分为模型组(10只)、西药组(12只)和温肾养血方大、中、小剂量(简称中药大、中、小剂量组,每组12只)。另选10只22日龄大鼠作为正常组。正常组和模型组每天给予蒸馏水灌胃。西药组给予枸橼酸氯米芬(0.58mg/100g)灌胃,连续5日。中药大、中、小剂量组分别给予温肾养血方5.2、2.6、1.3mg/100g灌胃,连续21日。采用放射免疫法检测大鼠促卵泡刺激素(follicle stimulating hormone,FSH)、促黄体生成素(luteinizing hormone,LH)、雌二醇(estradiol,E2)、孕酮(progesterone,P)和催乳素(prolactin,PRL)水平;酶免法检测抑制素(inhibin,INH)、激活素(activin,ACT)和卵泡抑素(follistatin,FS)含量。结果与正常组比较,模型组大鼠血清FSH、LH升高,P降低,差异均有统计学意义(P<0.05);模型组INH降低,FS升高,差异有统计学意义(P<0.05)。与模型组比较,西药组和中药大、中、小剂量组大鼠血清FSH均下降,西药组P下降,差异均有统计学意义(P<0.05);西药组和中药大、中、小剂量组INH均升高,FS均降低,中药大剂量组ACT升高,差异均有统计学意义(P<0.05)。结论温肾养血方可能是通过调节INH-ACT-FS系统和性腺激素水平促进卵泡发育的。

Objective To explore the effect of Wenshen Yangxue Recipe（WYR）on inhibin-activin-follistatin（INH-ACT-FS）system and gonadal hormone level in anovulatory rats.Methods Anovulatory rat model was established in 76 rats（9 days old）by subcutaneous injecting testosterone propionate（1.25 mg/0.05 mL for each rat）from the nape.Totally 58 successfully modeled rats were divided into 5 groups according to random digit table,i.e.,the model group（n=10）,the Western medicine（WM）group（n=12）,high,middle,and low dose WYR groups（n=12）.Besides,another ten 22-day old rats were recruited as a normal group.Distilled water was daily administered to rats in the normal group and the model group by gastrogavage.Clomiphene citrate（0.58 mg/100 g）was daily administered to rats in the WM group for 5 successive days.WYR at 5.2,2.6,1.3 mg/100 g was daily administered to rats in high,middle,and low dose WYR groups for 21 successive days.Levels of follicular stimulating hormone（FSH）,luteinizing hormone（LH）,estradiol（E2）,progesterone（P）,and prolactin（PRL）were detected using radioimmunoassay.Contents of inhibin（INH）,activin（ACT）,and follistatin（FS）were measured using ELISA.Results Compared with the normal group,serum levels of FSH and LH increased,and P level decreased in the model group（P〈0.05）;INH level decreased and FS level increased in the model group（P〈0.05）.Compared with the model group,serum FSH level decreased in the WM group and 3 WYR groups,P level decreased in the WM group（P〈0.05）;INH increased and FS levels decreased in the WM group and 3 WYR groups;ACT level increased in the high dose WYR group,with statistical difference（P〈0.05）.Conclusion WYR promoted follicular development possibly through regulating INH-ACT-FS system and gonadal hormone level.