固定化细胞发酵废蜜生产乳酸的研究

Lactic Acid Production from Molasse by Immobilized Lactobacillu delbrueckii

本文研究了海藻酸钙固定化德氏乳酸杆菌废糖蜜乳酸发酵的工艺条件。结果表明:采用硫酸酸化、聚丙烯酰胺加速絮凝的预处理方法产酸高;在不添加任何营养物时,乳酸转化率为72％;在添加适量酵母浸出汁、磷酸二氢钾、硫酸镁等无机盐时,乳酸转化率达80％,发酵液乳酸浓度达88g/l。

Batch fermentation by immobilized Lactobacillus delbrueckii in Ca—alginate was studied when molasses was used as substrate.The Pretreatment of sulphuric acid and flocculation of Polyacrylamide was the best.When only molasses was applied as substrate, the lactic acid yield was 72%.If the required nutrients, such as yeast extract, KH_2PO_4, MgSO_4 and MnSO_4,were added to the culture media, the yield was inccased up to 80%,the lactic acid concentration was 88g/l.