水稻粒形及千粒重QTL定位与上位性分析

QTL Mapping and Epistasis Analysis for Grain Shape and 1 000-grain Weight in Rice

利用粒形及千粒重存在显著差异的2个亲本（TY319、8B）杂交构建的F3群体为作图群体,对粒长、粒宽、长宽比及千粒重进行QTL定位与上位性分析。共检测出13个影响粒形及千粒重的QTL,分别位于第1,2,4,7,8,10,12染色体,LOD值介于2.66-5.88,表型变异贡献率介于7.41%-44.93%,其中,第1染色体上控制长宽比的q LWR-1未见报道,可能是新的QTL。此外,通过上位性分析,粒长、长宽比和千粒重3个性状共检测到21个互作,粒宽未检测到互作位点。

The QTL mapping and epistasis analysis were conducted on grain shape related traits including grain length （GL）, grain width （GW） and grain length-width ratio （LWR） and 1 000-grain weight （TGW） with the F3 population derived from the cross of TY319 and 8B, two parents significantly different in grain shape and TGW. There were 13 QTLs detected for grain shape and TGW, which were localized on Chromosome 1, 2, 4, 7, 8, 10 and 12 with their LOD values ranging from 2.66 to 5.88 and phenotypic variation estimation （PVE） from 7.41% to 44.93 %. Among them, the qLWR-1 on Chromosome 1 controlling LWR has not been reported, so it might be a new QTL for grain shape. Besides, there were 21 times of gene interaction detected to be related to the traits of GL, LWR and TGW, but no interaction found to be related to GW, based on the epistasis analysis.