熊果酸对成牙骨质细胞增殖及矿化功能的影响

Effects of Ursolic Acid on Proliferation and Mineralization of Cementoblasts

目的:观察熊果酸对鼠成牙骨质细胞系OCCM-30增殖、周期、凋亡及矿化功能的影响。方法:一定浓度熊果酸处理成牙骨质细胞后,cck-8法检测细胞增殖,流式细胞仪检测细胞周期及凋亡,微量酶标法检测碱性磷酸酶活性,茜素红染色法定量及定性检测矿化结节量。结果:熊果酸组(2.5μmol/L)相比于对照组,细胞增殖率和细胞周期增殖指数升高,早期凋亡率降低,碱性磷酸酶活性和矿化结节量上调。结论:一定浓度熊果酸可促进成牙骨质细胞增殖,G1期百分比降低,抑制早期凋亡,增强碱性磷酸酶活性,促进矿化结节的形成。

Objective：To investigate the effect of ursolic acid on murine cementoblasts cell line OCCM-30 in vitro.Methods：Cementoblasts were incubated with ursolic acid at various concentrations.Proliferation was detected using cck-8assay.Cell cycle and apoptosis were checked by flow cytometry analysis.The activity of alkaline phosphatase was tested by enzyme-linked method on microplate.The mineralization was tested by alizarin red S staining.Results：Compared with the control group,ursolic acid（2.5μmol/L）increased the cell proliferation rate and proliferation index of the cell cycle,reduced the early apoptotic rate,up-regulated the activity of alkaline phosphatase and the quantity of the mineralized nodules.Conclusion：Ursolic acid can promote the proliferation of cementoblast,enhance the activity of alkaline phosphatase,and facilitate the differentiation.