高效氯氟氰菊酯对大鼠皮层神经元钠通道的影响

Actions of Lambda-cyhalothrin on Sodium Channel in Rat Cerebral Cortical Neurons

[目的]研究旨在评价高效氯氟氰菊酯对哺乳动物的神经毒性作用。[方法]实验利用全细胞膜片钳技术研究高效氯氟氰菊酯对大鼠皮层神经元电压门控钠通道的影响。[结果]高效氯氟氰菊酯使钠电流的峰电压、稳态激活和失活曲线都产生超极化向移动,对最大峰电流和通道恢复没有显著性影响。[结论]高效氯氟氰菊酯与离子通道的开放状态结合力更强,从而影响其失活过程,但对钠通道动力学效应的影响却具有Ⅰ型菊酯特征。

[Aims] Voltage-gated sodium channel is important sites for the neurotoxic actions ofpyrethroid insecticides in mammals. The aim was to evaluate the neurotoxic effects of lambda-cyhalothrin. [Methods] We studied the mode of action of lambda-cyhalothrin on the native sodium channel in cerebral cortical neurons prepared from newborn rat brain, where the toxic effects are largely generated. [Results] Lambda-cyhalothrin caused a hyperpolarizing shift in steady-state activation and inactivation. No significant lambda-cyhalothrin-induced effect was observed at the peak current and the recovery from channel inactivation. [Conclusions] These results suggest that lambda-cyhalothrin binds to and modifies sodium channel in open state and exhibits the behavior of type I pyrethroids.