三种呼吸系统兽药制剂中非法添加盐酸溴己新、吲哚美辛的HPLC-PDA检测方法的建立

Determination of Bromhexine Hydrochloride and Indometcain Illegally Added in Three Veterinary Preparations by HPLC-PDA

为检测麻杏石甘散、氟苯尼考注射液中非法添加的盐酸溴己新，甘草颗粒中非法添加的吲哚美辛，以十八烷基键合硅胶为填充剂，磷酸盐缓冲液-乙腈（20：80）为流动相检测盐酸溴己新，以乙腈-0．1mol／L冰醋酸溶液（50：50）为流动相检测吲哚美辛，流速为1．0mL／min，波长扫描范围为200-400nm，建立了相应的HPLC-PAD检测方法，并采用峰纯度检查和光谱相似度检查辅助对照品比对方法，对非法添加药物进行确证。结果显示，麻杏石甘散和氟苯尼考注射液中盐酸溴己新的回收率分别为94．8％和98．5％，甘草颗粒中吲哚美辛回收率为95．2％；盐酸溴己新线性方程为Y=14780200x-16476，R=0．9999，吲哚美辛线性方程为Y=10995430x＋13033，R=0．9999；麻杏石甘散中盐酸溴己新检测限为83mg／kg，氟苯尼考注射液中盐酸溴己新检测限为1．6g／L，甘草颗粒中吲哚美辛检测限为1g／kg。

Methods for the determination of bromhexine hydrochloride in Maxing Shigan power and florfenicol injectionas well as indometcain in Gancao Granule were developed by the high performance liquid chromatography with photo -diode array detector（HPLC -PDA）. It was tested with C18 column, using phosphate buffer and acetonitrile （ 20 ： 80 ）, 0. 1 mol/L acetic acid and acetonitrile （ 50 ： 50 ） as the mobile phases. The scanning wavelength was from 200 to 400 nm. Peak purity test and spectrum similarity test were used to identify bromhexine hydroehloride and indometcain. The mean recoveries of bromhexine hydrochloridein maxing shigan power and florfenicol injection were 94.8% and 98.5%. The mean recovery of indometcain in Gancao granule was 95.2%. The linear equations of bromhexine hydrochloride and indometcain were y = 14780200x - 16476 （ R = 0. 9999 ） and y = 10995430x ＋ 13033 （ R = 0. 9999 ）. The limit of detections of blomhexine hydrochloride in Maxing Shiganpower and florfenicol injection were 83 mg/kg and 1.6 g/L. granule was 1 g/kg. In conclusion, the method is simple bromhexine hydrochloride and indometcain in three veterinary The limit of detection of indometcain in Gancao accurate and reliable for the determination of preparations.