四逆散对高脂血症小鼠血脂代谢途径的影响

Effects of Sini powder on blood lipid metabolism in mice with hyperlipidemia

目的探讨四逆散对高脂血症C57BL/6小鼠脂代谢的影响并初步探讨其机制。方法 40只SPF级C57BL/6小鼠作为研究对象,随机分为对照组、模型组、四逆散(0.9 mg/g体质量)组、枳实(0.9 mg/g体质量)组,分别以生理盐水、四逆散水煎剂、枳实水煎剂灌胃,干预时间为90天。全自动生化分析仪检测血清甘油三脂(triglyceride,TG),低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C),高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、ELISA法检测血清载脂蛋白A-1(apolipoprotein A-1,APOA-Ⅰ)水平及实时定量反转录-聚合酶链反应检测肝脏以及小肠组织中APOA-I的mRNA基因表达情况。结果在脂代谢方面,四逆散干预可显著防止高脂饮食小鼠血清TG、LDL-C水平的升高(P<0.05),显著提升血清中HDL-C和APOA-I水平(P<0.05),显著增加肝脏中APOA-I的mRNA表达程度(P<0.05)。结论四逆散可能是通过促进肝脏APOA-I mRNA表达和增加APOA-I分泌来干预血脂水平。

Objective To observe the effects of Sini powder on C57BL/6J mice with hyperlipidemia and explore its mechanism. Methods 40 C57 BL /6 mice were randomly divided into blank control group,hyperlipidemia group,hyperlipidemia plus Sini powder（ 0. 9 mg / g weight） group and hyperlipidemia plus fructus aurantii immaturus（ 0. 9mg / g weight）. Blank control group were fed with normal diet and the other groups were fed with high fat diet. Mice were received gavage administration with corresponding drug,Sini powder and fructus aurantii immaturus were intervened for 90 days. The automatic biochemistry analyzers method was used to measure the levels of TG,LDL-C,HDL-C in serum. Elisa methods were used to detect the APOA-I levels in serum. Real-time PCR methods were used to detect the APOA-I mRNA expression changes in liver and small intestine. Results Compared with hyperlipidemia group,Sini powder could decrease the levels of TG and LDL-C（ P 〈0. 05）,increase the levels of HDL-C and improve the level of APOA-I in serum（ P 〈0. 05）. The mRNA expressions of APOA-I in liver was increased in Sini powder combined hyperlipidemia group. Conclusion Sini powder may be to promote the expression of mRNA APOA-I in liver and increase APOA-I secretion to interfere with blood lipid level.