葎草提取物胃肠促动作用的实验研究

EXPERIMENTAL STUDY ON THE FUNCTION OF HUMULUS SCANDENS EXTRACT ON GASTROINTESTINAL ACTUATION

目的探讨葎草提取物对胃肠运动功能的影响。方法采用胃排空、肠推进运动实验法以及离体胃、肠实验法,研究葎草提取物对胃、肠运动功能的影响。结果蒸馏水组胃内容物残留率(%)和小肠推进比(%),分别为(82.53±10.69)和(50.88±8.68);葎草水提物大、中、小剂量(10.0、7.5、5.0 g生药/kg)组,胃内容物残留率(%)和小肠推进比(%),分别为(53.83±9.89)、(48.98±9.75)、(62.75±10.56)和(60.19±7.77)、(64.98±7.41)、(58.71±6.83);葎草醇提物中、小剂量(7.5、5.0 g生药/kg)组,胃内容物残留率(%)和小肠推进比(%),分别为(73.21±8.09)、(74.19±7.71)和(57.48±6.18)、(57.42±6.62)。与蒸馏水组比较,葎草水提物大、中、小剂量(10.0、7.5、5.0 g生药/kg)组均显著降低胃内容物残留率(P<0.01),明显提高小肠推进比(P<0.01,P<0.05);葎草醇提物中、小剂量(7.5、5.0 g生药/kg)组也明显降低胃内容物残留率(P<0.05),也明显提高小肠推进比(P<0.05);在降低胃内容物残留率及提高小肠推进比,葎草水提物与相同剂量的醇提物比较,差异显著(P<0.01)。在离体实验中,葎草水提物给药前后肠管、胃底环状肌的收缩幅度分别为(1.20±0.06)g、(72.01±4.27)mg和(4.89±0.60)g、(211.42±6.76)mg;葎草醇提物给药前后肠管、胃底环状肌的收缩幅度分别为(0.88±0.05)g、(70.20±3.26)mg和(1.43±0.17)g、(218.75±3.13)mg。与给药前比较,葎草水提物与醇提物给药后的肠管和胃底环状肌的收缩幅度明显强于给药前(P<0.01);从给药前后肠管收缩幅度差比较,葎草水提物与醇提物比较差异显著(P<0.01)。结论葎草提取物具有促进胃排空、肠推进运动的作用,且水提物作用强于醇提物。

objective： To explore gastrointestinal motor function of Humulus scandens extract. Methods： The gastric emptying, intestinal propulsion motor experimental method and experimental method of in vitro gastric and intestinal were used in studying the influence of the stomach, bowel movement function of water and alcohol extract from H. scandens. Results： The distilled water group, the gastric contents in residual rate （%） and the small intestine propulsion rate （%） were （82.53 ± 10.69）, （50.88± 8.68）; large-, medium- and small-dose （10.0, 7.5 and 5.0 g/kg） group of H, scandens water extract, the gastric contents in residual rate （%）and the small intestine propulsion rate （%） were （53.83 ±9.89）, （48.98 ±9.75）, （62.75 ±10.56） and （60.19 ± 7.77）, （64.98 ± 7.41）, （58.71 ± 6.83）; medium- and small-dose （7.5 and 5.0 g/kg） group ofH. scandens alcohol extract, the residual rate （%） of gastric contents and the small intestine propulsion rate （%） were （73.21 ± 8.09）, （74.19± 7.71） and （57.48 ± 6.18）, （57.42 ±6.62）. Compared with the distilled water group, large-, medium- and small-dose （10.0, 7.5 and 5.0 g/kg） group of H. scandens water extract significantly decreased the gastric contents in residual rate （P 〈 0.01）. Also obviously improved the small intestine propulsion rate （P 〈 0.01, P 〈 0.05）. Medium- and small-dose （7.5 and 5.0 g/kg） group of H. scandens alcohol extract significantly decreased the residual rate of gastric contents （P 〈 0.05）, also obviously improved the small intestine propulsion rate （P 〈 0.05）. Compared with the same dose of alcohol extract, water extract significantly decreased the residual rate of gastric contents and improved the intestinal propulsion rate （P 〈 0.01）. In vitro experiments, before and after the administration of water extract from H. scandens, the bowel and stomach bottom ring were （1.20 ± 0.06）g, （72.01 ±4.27）mg and （4.89 ± 0.60） g, （211.42 ±6.76） mg; before and after the administration of alcohol extract from H. scandens the bowel and stomach bottom ring were （0.88 ± 0.05）g, （70.20 ±3.26）mg and （1.43 ±0.17）g, （218.75 ±3.13） mg. the bowel and stomach bottom ring were significantly contracted after the administration of water and alcohol extract （P 〈 0.01）; from comparing the intestinal contraction amplitude difference before and after administration, the difference for water extract was more significant than that for alcohol extract （P 〈 0.01）. Conclusion： H. scandens extract possesses the role of promoting gastric emptying and intestinal propulsion motor, and the water extract has better effect than alcohol extract.