超连续谱激光对生物荧光激发效应的影响

Influence of Supercontinuum Laser on Bioluminescence Imaging Technology

针对荧光成像仪氙灯光源寿命短,激发能力弱的现状,提出了运用超连续谱激光(SCL)作为荧光激发光源的生物荧光成像技术;进行了荧光标准测试卡在两种光源激发下的荧光对比实验和不同SCL激发功率谱密度下的分析实验;通过线性拟合和外推的方法,对比分析了在相同曝光时间和功率下,两种光源的荧光激发能力;最后,通过对荧光基团在两种光源下的平均、最大光子密度流信息进行分析,进一步验证了实验结论:在生物荧光成像技术中,在相同激发功率谱密度下,相同的曝光时间内,SCL光源所激发的荧光强度是氙灯光源的2倍,且SCL具有更长的光源寿命和连续可调的光源参数,可满足荧光成像研究过程中对光源参数的不同需求,所做工作有利于解决荧光成像技术的光源瓶颈。

For the problem of weak exciting ability and short source life of xenon lamp in fluorescence imaging system, we proposed a new fluorescence excitation method that uses supercontinuum laser （SCL） to be the excitation source in bioluminescence imaging technology. The fluorescence imaging experiments of the fluorescence imaging test card by the two excitation sources were carried out and then the experiments at different SCL excitation power spectrum density were also completed. By the method of linear fitting and extrapolation, we analyzed the fluorescence excitation ability of the two sources at the same exposure time and power spectrum density. We obtain a conclusion that the fluorescence excitation intensity of SCL source is nearly twice of that of xenon lamp. Finally, through the analysis of the average and maximum photon density of the test perssad under two sources, the experiment results are proved again. The work is conducive to solve the problem of the source life and the fluorescence excitation ability of the light source in fluorescence imaging system.