超高效液相色谱串联质谱测定饮用水中PPCPs残留

Determination of Pharmaceuticals and Personal Care Products in Drinking Water by Ultra-high Performance Liquid Chromatography/Tandem Mass Spectrometry

建立了超高效液相色谱串联质谱（UPLC—MS／MS）同时测定饮用水中49种药物与个人护理品（PPCPs）残留的方法。采用Oasis HLB固相萃取柱对水样中的目标化合物进行富集净化，用液相色谱一串联质谱法进行分离和检测，以ACQUITY UPLC HSS T3色谱柱分离，以甲醇和0．1％甲酸水溶液为流动相进行梯度洗脱，质谱采用电喷雾正离子电离、多反应监测模式检测，内标法定量。结果表明，49种药物与个人护理品在生活饮用水中的三种不同加标量下的回收率分别为50．3％～124．5％、61．7％～123．3％和64．3％-124．6％，相对标准偏差（n=6）分别为1．1％-15.5％、0．3％～11．3％和0．9％～8．2％；最低检测质量浓度在0．05—5．73 ng／L之间。将建立的方法应用于北京某地表水、地下水以及市政水水样的分析，结果表明，该法操作简单、灵敏度高、选择性强，适用于饮用水中药物与个人护理品残留的测定。

An analytical method was developed to determine 49 pharmaceuticals and personal care products （PPCPs） in drinking water by ultra-high performance liquid chromatography/tandem mass spec- trometry （UPLC-MS/MS）. Water samples were enriched and purified with Oasis HLB SPE, separated through ACQUITY UPLC HSS T3 column, using 0. 1% formic acid solution and methanol as mobile phase, and detected by mass spectrometry in positive ion mode with multiple reaction monitoring using an electrospray ionization interface, quantified by an internal standard. The mean recoveries of the targeted 49 PPCPs spiked at three concentration levels were in the range of 50.3% to 124.5% , 61.7% to 123.3% and 64.3% to 124.6% , with relative standard deviations （RSDs） （n = 6） of 1. 1% to 15.5%, 0.3% to 11.3% and 0.9% to 8.2%. The method quantification limits （MQL） were in therange of 0.05 to 5.73 ng/L. The proposed method was used to determine the 49 PPCPs in ground water, tap water and surface water in Beijing. The method for the 49 representative PPCPs is simple, sensitive, specific, and suitable for the determination of trace contaminant residues in drinking water.