基质用量和料水比对蛹虫草子座虫草素产量的影响

Effect of Substrate Dosage and Substrate-to-Water Ratio on Yield of Cordycepin in Cordyceps militaris

探索基质用量和料水比对蛹虫草子座中虫草素产量的影响,为蛹虫草的优质栽培提供依据。以蛹虫草菌株CM-16为研究对象,小麦为主要栽培基质,采用单因素和二次正交旋转组合设计2种分析法,研究基质用量和料水比对蛹虫草子座中虫草素产量的影响。结果表明,随着基质用量和料水比的增加,蛹虫草子座中虫草素的产量均呈先增加后降低的趋势,当基质用量为每盒350g,料水比(质量体积比)控制在1∶1.5时,蛹虫草子座中虫草素质量分数最高,为3.96mg/g。基质用量和料水比是影响蛹虫草子座中虫草素质量分数的重要因素,当以小麦为主要原料栽培蛹虫草时,小麦用量为每盒350g,最佳料水比(质量体积比)控制在1∶1.5。

This study aims to investigate the effects of substrate dosage and substrate-to-water ratio in the medium on production of cordycepin in the stroma of Cordyceps militaris,and further provide scientific basis for C.militaris cultivation.Cordyceps militaris strain CM-16 and wheat were used as material and main cultivation medium,respectively.Single factor and two orthogonal rotation combination methods were used to test the effects of substrate dosage and medium substrate-to-water ration on cordycepin content in the stroma.The results showed that the increasing of substrate dosage and medium substrate-to-water ratio,the cordycepin content in stroma increased initially,then decreased.The cordycepin content in stroma reached its maximum：3.96 mg/g when the substrate dosage was350 g per box and medium substrate-to-water ration was 1∶1.5.In conclusion,the ubstrate dosage and medium substrate-to-water play an important role of the yield of cordycepin in stroma of C.militaris.When wheat was used as the main cultivation medium,the optimum substrate dosage and medium substrate-to-water ration were 350 g per box and 1∶1.5,respectively.