摘要
目的:研究汉滩病毒(HTNV)核蛋白细胞毒性T淋巴细胞(CTL)表位肽联合不同免疫佐剂免疫C57BL/6小鼠后的免疫学特性,确立一种免疫效果良好的多肽免疫C57BL/6小鼠方案。方法:分别用氢氧化铝、弗氏佐剂和脂质体作为免疫佐剂与汉滩病毒核衣壳蛋白上的CTL表位肽段混合,经皮下注射免疫C57BL/6小鼠,共免疫3次,每次间隔2周;免疫结束后分离小鼠脾细胞,并分别采用ELISPOT和CTL杀伤试验进行检测。结果:HTNV核蛋白CTL表位肽联合弗氏佐剂加脂质体组小鼠脾细胞分泌IFN-γ能力和CTL杀伤能力优于其他各实验组(P<0.01)。结论:HTNVCTL表位肽联合弗氏佐剂加脂质体免疫C57BL/6小鼠效果最佳,可为HTNV多肽疫苗的免疫策略提供参考。
Objective： To establish an improved immunization protocal to immune the C57BL/6 mice with epit-ope peptides through studying immunological characteristics of Hantaan virus（HTNV） nucleoprotein CTL epitopecombined with different immune adjuvants. Methods： C57BL/6 mice were subcutaneous immunized with HTNV nu-cleoprotein CTL epitope combined with aluminum hydroxide adjuvant, Freund＇s adjuvant, and immunoliposome, re-spectively. Immunization was performed three times, with 2 weeks interval. After immunization, the splenocytes ofmice were isolated to detect the immunological characteristics by ELISPOT and cytotoxicity assays. Results： Com-pared with other groups, group of CTL epitope combined with Freud＇s adjuvant and immunoliposome showed agreater ability to secrete interferon-γ and producted stronger cytotoxicity（P0.01）. Conclusion： HTNV nucleopro-tein CTL epitope combined with Freud＇s adjuvant and immunoliposome have an increased immune effect, provid-ing an alternative vaccination strategy.
出处
《生物技术通讯》
CAS
2016年第6期787-789,807,共4页
Letters in Biotechnology
基金
国家自然科学基金(31470890
31270978)
陕西省科技统筹创新工程计划(2013KTCL03-06)
军队重点课题(BWS13G024)
