藤茶泡水前后的抗菌活性和抗氧化活性比较

Antimicrobial Activity and Antioxidant Activity Comparison of Ampelopsis grossedentata before and after Soaking in Water

[目的]比较藤茶泡水前后乙醇提取物的抗菌活性和抗氧化活性。[方法]采用福林酚法进行酚含量的测定,抑菌圈法进行抗细菌活性测定,菌饼法进行抗真菌活性测定,并采用DPPH法进行抗氧化活性测定。[结果]藤茶泡水前后乙醇提取物的多酚含量分别为168和56 kg/g,藤茶泡水前乙醇提取物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、灰葡萄孢霉、胶孢炭疽菌和葡萄白腐病菌均有抑制作用,泡水后的乙醇提取物对枯草芽胞杆菌不再有抑制作用,对其他5种菌仍有抑制活性但活性均有不同程度的降低。藤茶泡水前乙醇提取物的DPPH清除活性IC50分别为5.09和36.01μg/m L。[结论]藤茶泡水后的残渣仍具有一定的抗菌和抗氧化活性,可被继续开发利用。

[Objective] To compare antibacterial activity and antioxidant activity of ethanol extracts from Ampelopsis grossedentata before and after soaking in water.[Method]The folin-ciocalteu method was used to make the total phenolic content, antibacterial and antifungal activities were done by the inhibition zone method and the fungus cake method respectively, and antioxidant activity was done by DPPH method.[Result] The total phenolic contents of ethanol extract of Ampelopsis grossedentata （ EEAG ） before and after soaking in water were 168 and 56 kg/g.EEAG before soaking in water showed antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Botrytis cinerea, Colletotrichum gloeosporioides and Coniothyrium diplodiella.EEAG after soaking in water exhibited no antimicrobial activity against Bacillus subtilis and lower antimicrobial activities against the other five microbes.The IC50 values of DPPH radical scavenging ability of EEAG before and after soaking in waer were 5.09 and 36.01 μg/mL respectively.[Conclution]There are certain antimicrobial and antioxidant activi-ties of the residue of Ampelopsis grossedentata after soaking in water, so it should still be exploited.