YDL080c基因缺失的低异戊醇酿酒酵母菌构建

Construction of Saccharomyces cerevisiae with Low Isopentanol Production by Deleting Gene YDL080c

[目的]构建YDL080c基因缺失的低异戊醇生成的酿酒酵母菌。[方法]利用Cre-lox P重组系统与酵母电转化法敲除酿酒酵母中编码类丙酮酸脱羧酶的基因YDL080c,切断酿酒酵母代谢中的异戊醇生成,从而构建一株低异戊醇生成的酿酒酵母菌株。[结果]成功构建出YDL080c基因缺失的工程酵母菌Y1,Y1酿造酒中相对异戊醇含量为0.81 g/L,比初始菌降低28.3%。[结论]利用基因敲除YDL080c的工程酵母菌能够有效减少酿造酒中异戊醇的生成量。

[Objective]The Saccharomyces cerevisiae with producing low isopentanol was constructed by knocking out geneYDL080c.[Method] The Saccharomyces cerevisiae with producing low isopentanol was constructed byusing Cre-loxP recombination system and yeast electrical trans-formation method to knock out the gene YDL080c encoding pyruvate decarboxylase,in order to cut off the produce of isopentanol in metabolism of Saccharomyces cerevisiae.[ Result] The engineering strain Y1 without YDL080c was successfully constructed.The relative isopentanol content was 0.81 g/L in wine brewed by Y1, which was 28.3% lower than that of the original strain.[Conclusion]Engineering Saccharomyces cerevi-siae without gene YDL080c can effectively reduce the production of isopentanol in brewed wine.