生石花植株离体再生及组培快繁研究

Plantlet Regeneration in vitro and Rapid Propagation of Lithops sp.

[目的]建立生石花植株离体再生及组培快繁体系。[方法]以生石花成熟种子为外植体,研究生石花组培快繁技术。[结果]萌发后的幼苗在MS+0.50 mg/L 6-BA+0.05 mg/L NAA培养基上被成功诱导出愈伤组织;不添加6-BA的MS培养基有利于愈伤组织的分化,不定芽诱导率为4.65;在MS+0.05 mg/L 6-BA+0.01 mg/L NAA培养基上不定芽增殖率较高,可达5.60。[结论]建立了生石花植株离体再生及快繁体系,有利于生石花种质资源保护和工厂化生产。

[Objective] The aim was to establish plantlet regeneration in vitro and rapid propagation of Lithops sp.[Method] With mature seeds as explant, rapid propagation technique of Lithops sp.was studied.[ Result] Calli were induced from the young seedling on MS medium ＋0.50 mg/L 6-BA＋0.05 mg/L NAA in this study.The results showed that the basic MS medium without 6-BA benefited the callus differentiation of Lithops sp., and the induction ratio of adventitious bud was 4.65;MS＋0.05 mg/L 6-BA＋0.01 mg/L NAA was the optimal medium for the multiplication of adventitious bud, and the multiplication ratio was 5.60.[Conclusion] The plantlet regeneration and rapid propagation system of Lithops sp.were established, which has important guiding significance for the germplasm protection and industrial production of Lithops sp..