黑羽鹌鹑ESRβ基因外显子多态性及其与产蛋性状的相关性分析

Single Nucleotide Polymorphisms in ESRβ Gene of Black Plumage Quail and Its Association with Egg Production

试验旨在研究鹌鹑雌激素受体β亚基(ESRβ)基因外显子多态性及其与产蛋性能的关联性,为鹌鹑产蛋性能候选基因的确立及分子标记辅助选育提供理论依据。选取健康蛋用黑羽鹌鹑为研究对象,开产后进行生产性能的持续统计(60d),采用PCR-SSCP法对鹌鹑ESRβ基因多态性进行检测,并运用SPSS 13.0统计软件进行ESRβ基因多态性与生产性能的相关性分析。PCR-SSCP检测结果发现,ESRβ基因外显子1、3、4、5、6、7和8均存在单核苷酸多态性。相关性分析结果表明,在开产蛋质量上,外显子3CD基因型与CC和DD基因型有显著性差异(P<0.05);在开产日龄上,外显子5DD基因型与CD和CC基因型有显著性差异(P<0.05);在平均蛋质量上,外显子7CD基因型与CC和DD基因型有显著性差异(P<0.05)。表明ESRβ基因与蛋用黑羽鹌鹑产蛋性状有一定关联性。

This study aimed to investigate the correlation between ESRβgene exon polymorphism and egg production of Black plumage egg quail,and provide the theoretical foundation for molecu-lar selection.The healthy Black plumage egg quail were selected as research object,and the egg production were analyzed using SPSS 13.0 software.Estrogen receptor β（ESRβ）subunit gene was selected as candidates gene to screen the genomic variation by PCR-SSCP,and the association of the ESRβ gene polymorphisms with egg production were analyzed.The results of polymor-phisms detection showed that exon 1,exon 3,exon 4,exon 5,exon 6,exon 7 and exon 8 of ESRβgene all had nucleotide variation.The association analysis results of ESRβ gene exons and egg production showed that the egg weight of first laying of exon 3 CD genotype were significantly higher than that of CC and DD genotypes （P 〈0.05）.The age at first laying of exon 5 was also significant difference between DD and CC,CD genotypes （P 〈0.05）.Moreover,the average egg weight of exon 7 CD genotype was significant higher than that of CC and DD genotypes （P 〈0.05）.These data suggested that ESRβ gene could significantly affect egg production of Black plumage egg.