上海市肉鸽沙门氏菌的分离鉴定及流行血清型和耐药性分析

Isolation,Identification and Analysis of Serotype and Drug Resistance of Salmonellafrom Pigeon in Shanghai

为了解上海市农贸市场肉鸽群体中沙门氏菌流行血清型和耐药情况,本试验于2011—2014年间从上海市各区农贸市场采集肉鸽新鲜粪便样本92份,用XLD平板和沙门氏菌属显色培养基分离疑似沙门氏菌,革兰氏染色镜检并进行生化试验,共获得沙门氏菌24株,分离率为26.1%。采用Kauffmann-White法和K-B纸片法对24株沙门氏菌进行血清型鉴定和16种抗生素的药敏试验。血清型鉴定显示,24株沙门氏菌可分为鼠伤寒(66.7%)、阿贡纳(25.0%)和科瓦利斯(8.3%)3种血清型。药敏试验结果显示,有75.0%(18株)的分离株表现出不同程度耐药,其中分离株对四环素和磺胺异唑耐药率最高,均达到62.5%,其次为链霉素(58.3%)、萘啶酸(50.0%)、氨苄西林(20.8%)。多重耐药菌株15株(62.5%),耐4种抗菌药物的菌株最多,占16.7%(7株)。另外,分离菌株对头孢吡肟、头孢噻肟、头孢噻甲羧肟、亚胺培南、氧氟沙星等5种药物的敏感率为100.0%,对奥格门丁和环丙沙星有12.5%的中介敏感率。本研究结果表明,上海市肉鸽中沙门氏菌的携带率较高,且菌株多重耐药现象较严重,这为食品中沙门氏菌的防控工作带来较大的挑战和风险,肉鸽沙门氏菌的流行和耐药情况应值得密切关注。

The purpose of this study was to investigate the molecular types and antimicrobial re-sistance of Salmonella isolated from pigeon in Shanghai from 201 1 to 2014.A total of 92 fecal samples were collected from markets.XLD plate and Salmonella chromogenic medium were used to isolate suspected Salmonella colonies,and then determined the number of Salmonella through gram staining and biochemical tests.Among them,24 （26.1%）were positive for Salmonella . Kauffmann-White method and the K-B method were used respectively for serotype identification and susceptibility testing.In result,serological identification showed that 24 isolates from pigeon could be divided into 3 serotypes including S .typhimurium （66.7%）,S .agona （25.0%）and S .corvallis （8.3%）.Drug susceptibility test showed that 75.0% of the isolates were resistant to one antibiotic at least.The highest level resistance were found for tetracycline as well as sulfisox- 〈br〉 azole （62.5%）,followed by streptomycin （58.3%）,nalidixic acid （50.0%）and ampicillin （20.8%）.Multi-drug resistant strains accounted for 62.5% （15）,in which the largest number of strains （7,1 6.7%）were resistant to four drugs.In addition,isolates were 100.0% susceptible to cefepime,cefotaxime,ceftazidime,imipenem and ofloxacin,but 12.5% were moderately sen-sitive to the amoxicillin/clavulanic acid and ciprofloxacin.The study showed that Salmonella had a high separation rate of pigeon in Shanghai farmers market and performed serious multidrug re-sistance,which would bring great challenges and risks to the prevention and control of Salmonel-la in food,so that the prevalence and drug resistance of Salmonella in pigeon deserved close at-tention.