生殖道沙眼衣原体感染相关的生物学标志评估

Evaluation of biological markers associated with Chlamydia pneumoniae infection in genital tract

目的探讨可以评估生殖道沙眼衣原体感染相关的生物学指标。方法选取2014-08—2015-12间诊断为生殖道沙眼衣原体感染的32例育龄妇女为感染组。选取同期无感染的32例育龄妇女为未感染组。采用ELISA法对比2组HD-5、TNF-α、IL-8、IL-1α、IL-6的表达。同时利用脂多糖(LPS)刺激体外培养的正常宫颈上皮细胞,对比LPS刺激后细胞与正常细胞不同时间点的HD-5、TNF-α水平变化。结果感染组HD-5、TNF-α、IL-8、IL-1α、IL-6表达均显著高于未感染组。LPS刺激后细胞在感染1d、2d后的HD-5、TNF-α水平均显著高于正常细胞,差异均有统计学意义(P<0.05)。结论沙眼衣原体属于生殖道感染的重要病菌,应注意检查及预防。

Objective To explore the biological marker of Chlamydia infection in genital tract. Methods In our hospital diagnosed as genital Chlamydia trachomatis infection of childbearing age women in 32 cases from the August 2014 to December 2015, remembered as the infection group. The other non reproductive tract infections 32 cases of women of childbearing age remember as the uninfected group. Using ELISA method and compared between the two groups HD - 5, TNF - α, IL - 8 level and IL - 1 α, IL - 6 content.＇ At the same time, the lipopolysaccharide in vitro stimulation of normal cervical epithelium under the package, compared with the LPS stimulated cells and normal cells at different time points of HD - 5, TNF -α. Results The levels of HD - 5, TNF - α, IL - 8, IL - 6 and IL - 1α were significantly higher in the infection group than those in the uninfected group, and the levels of the infection group were significantly higher. After LPS stimulation, the levels of HD - 5, TNF - IL - 6 and 1 d in LPS group were significantly higher than those in the normal group after infection with 2d. Conclusion Chlamydia pneumoniae is an important pathogen of reproductive tract infection, and should be paid attention to and prevention.