基于抗骨质疏松作用的淫羊藿拆分组分的筛选研究

Study on Filtrating Separate Composition of Epimedii Folium Based on Its Anti-osteoporosis

目的:研究淫羊藿及其拆分组分对维甲酸致骨质疏松模型大鼠的影响,筛选最有效组分。方法:考察淫羊藿各组分对维甲酸致骨质疏松模型大鼠血清中Ca^(2+)、P^(3+)、ALP、Str ACP、BCG、E2的含量变化以及对骨钙、骨磷、双侧腿骨骨质量/体质量比、单侧股骨灰质量/干质量比的影响,并对股骨进行骨组织病理学观察。结果:淫羊藿30%乙醇组分可显著增加模型大鼠骨钙、骨磷含量和血清E2水平,降低血清ALP、Str ACP、BCG及磷含量,显著提高双侧腿骨质量/体质量比和单侧股骨灰质量/干质量比;淫羊藿全成分可显著增加骨钙、双侧股骨骨质量/体质量比和单侧股骨灰质量/干质量比,降低血清ALP及磷含量;骨组织形态学观察结果显示,除阳性对组外,淫羊藿30%乙醇段对骨病理形态改善最明显,可明显增加骨小梁的密度和间隙,仅局部骨壁呈间断性松质骨结构;综合各指标得出各组分抗骨质疏松作用大小依次为:淫羊藿30%乙醇段>淫羊藿全成分>淫羊藿水段>淫羊藿95%乙醇段>淫羊藿多糖。结论:淫羊藿各拆分组分中淫羊藿30%乙醇段在抗骨质疏松方面作用最显著,淫羊藿全成分也有较好的抗骨质疏松作用,但效果不如淫羊藿30%乙醇段,淫羊藿水段和淫羊藿95%乙醇段对骨质疏松可能有一定的预防作用,淫羊藿多糖的抗骨质疏松作用不明显。

Objective： To investigate the pharmacological effects of Epimedii Folium and its different extacted constituents on the rats with RA-induced osteoporosis. To screening the most active constituent. Methods： With building animal model of osteoporotic rat induced by RA,different constituents extracted from Epimedii Folium affected the content of Ca^2 ＋,P^3 ＋,ALP,Str ACP,BCG and E2 in serum were measured,and on the calcium and phosphate of bone,mass-ratio of in bilateral femur,ash to dry ratio in unilateral were evaluated. The thigh-bone morphological was observed through histomorphology. Results： The 30% alcohol section can significantly increase the content of Ca^2 ＋,P^3 ＋in model rats,promote the level of E2 in serum,and decrease the content of Str ACP,BCG and P3 ＋in serum,significantly raise the mass-ratio of in bilateral femur,and ash to dry in unilateral. The entire ingredient section can significantly increase Ca^2 ＋,mass-ratio of in bilateral femur,and ash to dry in unilateral,decrease ALP and P3 ＋in serum. In comparing with other groups,except for the positive control group,the observation of histomorphology exhibit that the 30% alcohol section improved the pathomorphism of bone most obviously and significantly increased the density and space of trabecular,with discontinuous spongy bone existing in local gusongzhiincus buttress. The sequence for anti-osteoporosis was 30% alcohol section entire ingredien water section 95% alcohol section polysaccharide. Conclusion： The 30% alcohol section manifests the most significant effect on anti-osteoporosis. The entire ingredient section also has a certain degree of effect on anti-osteoporosis but weaker than 30% alchol section. The water and 95% alcohol sections show a certain extent of preventive effect on anti-osteoporosis,and the effects of polysaccharide group are inapparent.