海参极性脂质对东莨菪碱致痴呆小鼠学习记忆能力的保护作用

Protective Effects of Sea Cucumber Polar Lipids on Dementia Induced by Scopolamine in Mice

目的:研究海参极性脂质对东莨菪碱模型小鼠学习记忆能力的影响,探讨其可能的作用机制。方法:将小鼠随机分为4组:正常对照组、东莨菪碱模型组、海参磷脂(SCP)组和海参脑苷脂(SCC)组。各组小鼠喂食正常饲料,SCP组添加0.5%SCP,SCC组添加0.05%SCC,连续喂养7 d。于第8天造模,正常对照组灌胃生理盐水,其余各组腹腔注射5 mg/kg东莨菪碱。采用Morris水迷宫实验测试小鼠的学习记忆能力,连续7 d。行为测试结束后,对小鼠脑组织进行超氧化物歧化酶(SOD)、丙二醛(MDA)、乙酰胆碱酯酶(TChe)测定。结果:Morris水迷宫实验中,与模型组小鼠相比,SCP和SCC组小鼠潜伏期均缩短,总路程减少,目标象限停留时间比例增加,穿越平台次数增加;SCP和SCC组小鼠脑组织TChe和MDA相对于模型组明显降低,SOD明显增加。结论:海参极性脂质对东莨菪碱致痴呆小鼠的学习记忆能力具有一定的改善作用,其作用机制可能与抗氧化和提高胆碱能有关。

Objective： To investigate the protective effects of sea cucumber polar lipids on scopolamine-induced de- mentia in mice and its possible molecular mechanisms. Methods： Mice were randomly divided into 4 groups： normal control group, scopolamine model group, sea cucumber phospholipid （SCP） group, sea cucumber cerebroside （SCC） group. The mice were fed on chow diet for 7 days, SCP group was added 0.5% SCP, SCC group was added 0.05% SCC. Make mold at the 8th day, the normal group was treated with physiological saline, other groups were injected with 5 mg/kg scopolamine. Learning and memory ability were tested by Morris water maze for 7 days. After the test, the con- tent of SOD, MDA and TChe in brain tissue were measured. Results： In Morris water maze test, compared with model group, treatment with SCP and SCC decreased the escape latency and total distance, increased the time spent in the platform quadrant and the number of crossing times. SCP and SCC could significantly decrease the content of MDA and TChe, increase the activities of SOD in brain tissues. Conclusions： Sea cucumber polar lipids can protect the ability of learning and memory of scopolamine-induced dementia mice and the mechanisms may be related to antioxidation and the increased cholinergic.