摘要
目的探讨结核感染T细胞斑点试验(T-SPOT.TB)技术联合胸水腺苷脱氨酶(ADA)和痰液结核杆菌(TB-DNA)测定对结核性胸膜炎的定性诊断,以达到早期诊断的目的。方法选取唐都医院2014年7月-2015年12月收治的86例结核性胸膜炎患者作为试验组,80例非结核性胸膜炎患者作为对照组,均行T-SPOT.TB检测,同时联合检查患者胸水ADA和痰液TB-DNA,比较T-SPOT.TB、ADA和TB-DNA检测与三者联合检测的应用价值。结果 86例结核性胸膜炎患者,T-SPOT.TB阳性率为84.88%(73/86),ADA阳性率为53.48%(46/86),痰液TB-DNA阳性率为31.40%(27/86),三者联合检测阳性率为93.02%(80/86)。结论 T-SPOT.TB检测联合ADA和痰液TB-DNA检测对结核性胸膜炎的诊断有较高的敏感性,对疑似结核性胸膜炎患者的快速、准确诊断具有重要的辅助诊断价值。
Objective To discuss the qualitative diagnosis of tuberculous pleurisy under T cell spot test of TB infection （T-SPOT.TB） combined with ADA and real-time fluorescent quantity PCR or detection of bacillus tuberculosis DNA（TB-DNA） to achieve early diagnosis and treatment purpose. Methods We selected 86 patients of tuberculous pleurisy as the observation group and 80 patients of non-tuberculous pleurisy as the control group received treatment in our hospital from July 2014 to December 2015, and gave them T-SPOT.TB detection and at the same time, did the joint inspection of ADA and TB-DNA levels and compared with the results of the combined determination of three administered simultaneously to evaluate the clinical value. Results Positive rate of T-SPOT.TB, ADA, sputum TB-DNA determination and the combined determination of three for 86 patients with tuberculous pleurisy was 84. 88% （ 73/86 ） , 53. 48% （ 46/86 ） , 31. 40% （ 27/86 ） and 93. 02% （ 80/86 ） respectively. Conclusion The combined detection of T-SPOT.TB, ADA and sputum TB-DNA has a higher sensitivity in diagnosis of tuberculous pleurisy, and has important auxiliary diagnostic value for patients with suspected tuberculous pleurisy.
出处
《东南国防医药》
2016年第5期483-485,504,共4页
Military Medical Journal of Southeast China
