控制哈茨木霉菌体形态提高β-1,3-葡聚糖内切酶活的研究

Enhanced endo-β-1,3-glucanase produciton through morphology control of Trichoderma harzianum

哈茨木霉的深层培养菌体形态与β-1,3-葡聚糖内切酶酶活之间有密切关系。通过单因素试验优化了控制哈茨木霉菌球形态的培养条件为:摇瓶装液量100 m L/500 m L,接种量5%(V/V),初始p H 6.5,回旋式摇床转速180r/min,在培养基中添加0.3%(m/V)的吐温-80或0.3%(m/V)的CMC-Na。在该条件下,菌球直径控制在(0.42±0.09)mm左右,β-1,3-葡聚糖内切酶酶活达到(111.9±7.5)U/m L。进一步通过正交优化,β-1,3-葡聚糖内切酶酶活提高到了135.2±8.3 U/m L,同时得出各种因素对菌球直径的影响顺序为:接种量>CMC-Na>吐温-80。

There is a close relationship between morphology of Trichoderma harzianum and endo-β - 1,3-glucanase production. The culture conditions for good morphology of Trichoderma harzianumin was optimized by single factor experiment, and the result showed as follow： 100 mL medium in 500 mL Erlenmeyer flask, 5% （V/V） of inoculum volume. The initial pH of the medium was 6. 5 and sha- king speed was 180 r/min, 0. 3% （m/V） Tween -80 or 0. 3% （m/V） CMC-Na was added into the medium as dispersing agents. The mycelia pellet diameter was about （0. 42 ± 0. 09 ）mm and the activity of endo-β - 1,3-glucan was （ 111.9 ±7. 5 ） U/mL. In the further orthogonal optimization, the activity of endo-β - 1,3-glucan reached （ 135.2 ± 8.3 ） U/mL, and the influence order of culture conditions to mycelia pellet diameter was inoculum volume 〉 CMC-Na 〉 Tween - 80.