液相色谱-质谱法检验人血浆中的沙丁胺醇

Determination of Salbutamol in Human Plasma by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

目的 建立人血浆中沙丁胺醇的液相色谱-质谱联用（UPLC-MS/MS）检验方法。方法 采用HSS T3色谱柱（2.1 mm×50 mm,1.7μm）,流动相为甲醇-0.1%甲酸水,梯度洗脱,流速为0.6 m L/min,采用UPLCMS/MS方法测定血浆中沙丁胺醇。结果 人血浆中沙丁胺醇在1~1000 ng/m L范围内与峰面积比呈良好线性关系,最低检测限为0.1 ng/m L,方法回收率大于70%,日内及日间精密度均小于10%。结论 经过多起案件的验证,所建立的方法简便、易于操作,且灵敏度高,能用于血浆中沙丁胺醇的检验。

Objective To develop a UPLC-MS/MS method for determining salbutamol in human plasma. Methods The separation of salbutamol was performed on a column of HSS T3 （ 2.1 mm×50 mm, 1.7 μm ） . The raobile phase consisted of water solution containing 0.1% formic acid and methanol. The flow rate was 0.6 mL/min with gradient elution. Salhutamol in human plasma was determined by UPLC-MS/MS. Results The standard curve was linear over the range of 1-1000 ng/mL, and the lowest detectable limit was 0.1 ng/mL. The absolute recovery was more than 70 %. Both of the intra- and interday precision was within 10 % at three concentrations. Conclusion The procedure was fully validated in terms of sensitivity, selectivity, precision and accuracy. The method developed was applied to the determination of salbutamol in human plasma samples collected from salbutamol-poisoning cases.