MK-1、RegⅣ在胆囊腺癌组织的表达及临床意义的研究

Expression and clinical significance of Reg and MK-1 in gallbladder adenocarcinoma

目的：探讨MK-1抗原（MK-1）和再生基因蛋白Ⅳ（RegⅣ）在胆囊腺癌组织中的表达及临床意义。方法：选取2001年1月-2014年6月我院胆囊手术切除的标本，其中胆囊腺癌87例，癌旁组织87例，慢性胆囊炎57例。采用免疫组织化学方法检测各标本MK-1和RegⅣ蛋白的表达。结果：胆囊腺癌组织中MK-1和RegⅣ表达阳性率分别为62．07％和56．32％，明显高于癌旁组织（20．69％、22．98％）和慢性胆囊炎组织（14．04％、10．53％），差异有统计学意义（P〈0．05）；胆囊腺癌组织中MK-1、RegⅣ蛋白表达与分化程度、淋巴结转移、周围组织浸润显著相关（P〈0．05）。87例胆囊腺癌患者中52例获得随访，其中术后生存期≥1年者19例，术后生存期〈1年者33例，平均生存期8．64±4．29个月。经Kaplan—Meier分析，MK-1表达阳性患者术后平均生存期明显高于阴性表达患者（χ2=4．750，P=0．029），RegⅣ表达阳性患者术后平均生存期明显低于阴性表达患者（χ2=4．244，P=0．029）。结论：MK-1和RegⅣ表达与胆囊腺癌局部侵袭、淋巴结转移及分化程度相关，可能成为预测患者生存期的重要指标。

Objective： To investigate the expression and clinical significance of MK-1 （Reg） and regenerative gene protein（Ⅳ） in gallbladder adenocarcinoma. Methods： Selected January 2001 - June 2014 in our hospital gallbladder surgical resection specimens, 87 cases of gallbladder adeno-carcinoma, 87 cases of adjacent cancer tissue, 57 cases of chronic cholecystitis. Immunohistochemical method was used to detect the expression of MK-1 and RegⅣ protein in different samples. Results： In gallbladder adenocarcinoma MK-1 and reg IV expression positive rate of 62.07% and 56.32%, was significantly higher than that in paracancerous tissue 20.69%, 22.98% and chronic cholecystitis 14.04%, 10.53% and the difference had statistical significance （P〈0.05）; The expression and differentiation degree, lymph node metastasis, and infiltration of Reg IV and MK-1 were significantly related to the expression of and in gallbladder adenocarcinoma （P〈0.05）; 87 cases of gallbladder cancer patients, 52 patients were followed, in which the survival of≥ 1 year in 19 cases, postoperative survival 〈1 year in 33 patients, with an average survival time of 8.64 ± 4.29 months. By Kaplan-Meier analysis, MK-1 expression positive patients, the average survival time was significantly higher than that of negative expression patients（ χ 2=4.750, P=0.029）, Reg Ⅳ expression positive patients, the average survival time was significantly lower than that of negative expression patients（χ2=4.244, P=0.029）. Conclusion： The expression of MK-1 and Reg IV is related to the local invasion, lymph node metastasis and differentiation degree of gallbladder adenocarcinoma, which may be an important index to predict the survival time of patients.