高浓度葡萄糖对人胃腺癌SGC-7901细胞增殖迁移和侵袭的影响

Effects of high glucose on proliferation,migration and invasion of gastric gland carcinoma SGC-7901 cells

目的探讨高浓度葡萄糖(高糖)对人胃腺癌SGC-7901细胞增殖、迁移和侵袭的影响。方法体外培养人胃腺癌SGC-7901细胞,在不同浓度葡萄糖(5.5、11.0、22.0 mmol/L)培养基进行处理,采用四甲基偶氮唑蓝比色法(MTT)检测SGC-7901细胞的增殖率,采用划痕实验检测SGC-7901细胞的迁移指数(MI),采用Transwell实验检测SGC-7901细胞的侵袭力。结果24、48、72 h各时间点5.5、11.0、22.0 mmol/L葡萄糖组光密度值均有不同程度上升,22.0、11.0mmol/L葡萄糖组明显高于5.5 mmol/L葡萄糖组,差异均有统计学意义(P<0.05),随葡萄糖浓度的增高和作用时间的延长,SGC-7901细胞增殖率逐渐增大。5.5、11.0、22.0 mmol/L葡萄糖组24 h MI分别为0.394±0.058、0.510±0.044、0.615±0.039,48 h MI分别为0.548±0.058、0.685±0.009、0.782±0.038;随葡萄糖浓度的增高和作用时间的延长,SGC-7901细胞MI逐渐增大,22.0、11.0 mmol/L葡萄糖组明显高于5.5 mmol/L葡萄糖组,差异均有统计学意义(P<0.05)。5.5、11.0、22.0 mmol/L葡萄糖组的穿膜细胞数量分别为(45±5)、(71±4)、(90±10)个,随葡萄糖浓度的增高,发生侵袭的SGC-7901细胞数量逐渐增多,22.0、11.0 mmol/L葡萄糖组明显高于5.5 mmol/L葡萄糖组,差异均有统计学意义(P<0.05)。结论高糖环境可促进SGC-7901细胞增殖,提高细胞迁移、侵袭能力。

Objective To investigate the effects of high glucose on the proliferation,migration and invasion of gastric gland carcinoma SGC-7901 cells. Methods SGC-7901 cells were cultured in vitro and treated by medium at different glucose concentrations of 5.5,11.0, 22.0 mmol/L. MTT method was applied to detect the proliferation rate,the scratch test was performed to test the migrate index（MI）,and the Transwell test was used to evaluate the invasiveness. Results The optical density（OD）values were increased in each time point（24,48,72 h） and each glucose group（5.5,11.0, 22.0 mmol/L）. The OD values in 24,48,72 h in the 22.0,11.0 mmol/L glucose groups were obviously higher than those in the 5.5 mmol/L glucose group,the differences were statistically significant（P〈0.05）. With the glucose concentration increase and action time prolongation,the SGC-7901 cell proliferation rate was increased gradually. The 24 h MI in the 5.5,11.0,22.0 mmol/L glucose groups were 0.394 ±0.058,0.510 ±0.044 and 0.615 ±0.039 respectively,and the 48 h MI were 0.548 ±0.058,0.685 ±0.009 and 0.782 ±0.038 respectively. With the glucose concentration increase and action time prolongation,the SGC-7901 cell MI was increased gradually. The MI in the22.0,11.0 mmol/L glucose groups were obviously higher than those in the 5.5mmol/L glucose group,the difference was statistically significant（P〈0.05）. The numbers of transmembrane cells in the 5.5,11.0 and 22.0 mmol/L glucose groups were 45±5,71±4 and90±10 respectively. With the increase of glucose concentration,the invasive SGC-7901 cells number was increased gradually. The invasive cells number in the 22.0 and 11.0 mmol/L glucose groups were obviously higher than those in the 5.5 mmol/L group glu cose group,the difference was statistically significant（P〈0.05）. Conclusion The high glucose environment could promote the proliferation of SGC-7901 cells,and improve their migration and invasion abilities.