畜禽养殖场空气中可培养抗生素耐药菌污染特点研究

Pollution Characteristics of Antibiotic Resistant Bacteria from Atmospheric Environment of Animal Feeding Operations

畜禽养殖场被认为是空气环境中耐药基因和致病菌的重要来源.本研究对北京地区22个畜禽养殖场逸散细菌、四环素和红霉素耐药菌气溶胶的浓度进行检测,对其粒径分布和动力学粒径进行分析.结果表明,所调查的3种生物气溶胶(细菌、四环素耐药菌和红霉素耐药菌),在猪舍内浓度最高,牛舍内最低.蛋鸡舍内两种抗生素耐药菌气溶胶的浓度均低于肉鸡.本研究在蛋鸡和肉鸡舍外空气中检测到了四环素和红霉素耐药菌,所占丰度分别为8.81%、15.89%和23.19%、36.53%.不同养殖场舍内外细菌、四环素耐药菌和红霉素耐药菌气溶胶浓度的粒径分布特点存在差异.动力学粒径研究结果显示,4种动物舍内的四环素和红霉素耐药菌气溶胶主要沉降在人体的咽喉和支气管.本研究结果将为评价养殖场生物气溶胶对周边空气环境污染及人类健康造成的危害提供基础数据.

Animal feeding operations is considered as a source of airborne human pathogens and antibiotics genes. In this study,we investigated the concentration,size distribution and aerodynamic diameter of airborne bacteria,tetracycline resistant bacteria and erythromycin resistant bacteria from 22 animal feeding operations in three districts of Beijing. The results indicated that the highest and lowest concentrations of these three types of bioaerosol were detected in pig and cow house,respectively. The concentration of airborne bacteria was higher in layer house,whereas concentration of antibiotic resistant bacteria was higher in broiler house. Tetracycline and erythromycin resistant bacteria were detected from the atmospheric environment from out door of layer and broiler house with the percentages of 8. 81%,15. 89% and 23. 19%,36. 53%,respectively. The size distribution and aerodynamic diameter varied from inside and outside of pig,cow,and layer and broiler house. Most of tetracycline resistant bacteria and erythromycin resistant bacteria from current four types of animal feeding operations mainly deposited in pharynx,larynx and bronchus of respiratory system. This study would offer the base data for evaluating the detriment of bioaerosol from animal feeding operations to the worker and environment.