摘要
目的探讨选择性肠道去污染对改善大鼠90%肝切除术预后的影响。方法sD大鼠分为选择性肠道去污染(SBD)组和对照组,分别在术前使用庆大霉素、多黏菌素、制霉菌素的三联抗生素和等量生理盐水进行灌胃,随后均接受90%肝切除术(SHx)。术后采用多种方法对血清脂多糖(LPS)、肝功能、肝脏组织损伤、肠黏膜屏障损伤、炎症相关信号通路及肝再生等指标进行检测方法。结果90%肝切除术可显著提高大鼠循环血内的LPS水平。术前SBD可抑制肠道革兰氏阴性菌的生长、改善肠道黏膜屏障损伤、降低内毒素血症的发生率;减轻肝功能损害,减少肝实质炎症反应,改善肝再生,从而改善预后。结论术前SBD可显著改善极限肝切除术的预后。
Objective To investigate the effect of selective bowel decontamination (SBD) on prog- nosis of 90% hepatectomy in rats. Methods We adopted rat model of subtotal hepatectomy(90%, SHx), gentamicin + polymyxin + nystatinor saline of the same amount was administrated preoperatively. Liver damage makers, portal and systemic lipopolysaccharide(LPS) , mucosal damage, signaling pathways and liv- er regeneration were investigated. Results We found that SHx resulted in significantly enhancedsystemic LPS. Inhibition of gastrointestinal gram-negative bacteria by SBD significantly reduced LPS levels and im- proved survival after SHx. SBD protected intestinal mucosa barrier, alleviated liver parenchymal damage and inflammation and promoted liver regeneration. Conclusion SBD is beneficial and necessary for extended heptactomy.
作者
任为正
苏茂生
杜晓霞
赵莉
吴小忠
顾万清
Ren Weizheng Su Maosheng Du Xiaoxia Zhao Li Wu Xiaozhong Gu Wanqing(Department of Hepatobiliary Surgery, PLA General Hospital, Beijing 100853, China)
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2016年第12期843-847,共5页
Chinese Journal of Hepatobiliary Surgery
基金
全军医药卫生科研基金课题(11BJZ13)
关键词
选择性肠道去污染
内毒素
极限肝切除
肝再生
预后
大鼠
Selective bowel decontamination
Endotoxin
Extended hepatectomy
Liver re- generation
Prognosis
Rat