外源GA_3对盐胁迫下板蓝根种子萌发及幼苗生理生化指标的影响

Effects of Exogenous GA_3 on Seed Germination and Physiological and Biochemical Indexes of Seedling of Radix isatidis Under Salt Stress

以板蓝根品种安徽亳州种为试材,在100 mmol/L Na Cl盐胁迫处理下,研究了外源GA_3对盐胁迫下板蓝根种子萌发及幼苗生理生化指标的影响。结果表明:当外源GA_3的浓度达到100 mg/L时,板蓝根种子萌芽能力和幼苗生长表现最优;同时,幼苗光合速率、蒸腾速率、气孔导度和胞间CO_2浓度的数值最大,分别为CO_2 5.53μmol/(m^2·s)、0.56 mmol/(m^2·s)、H_2O 216 mmol/(m^2·s)和287 mg/L;幼苗叶片中MDA含量达最低,而POD和SOD活性最高,其值分别为0.336μmol/g FW、26.65 U/(g·min)FW和272.53 U/(g·min)FW,可有效缓解盐胁迫对板蓝根种子萌发和幼苗生长造成伤害。

Taken the varieties of Radix isatidis from Bozhou in Anhui Province as experimental material, under 100 mmol/L NaC1 salt stress, the effects of exogenous GA3 were studied on seeds germination and seedling physiological biochemical indexes. The results showed that when the concentration of exogenous GA3 100 mg/L, seeds germination and seedlings growth were optimal; at the same time, photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO2 concentration reached the maximum values, were CO2 5.53 pmol/（m2·s）, 0.56 mmol/（m2·s）, H20/216 mmol/（m2·s） and 287 mg/L, respectively; MDA content in seedling leaves was the lowest while the activities of POD and SOD were the highest, were 0.336 μmol/g FW, 26.65 U/（g·min） FW and 272.53 U/（g·min） FW, respectively, which can effectively alleviate salt stress damage to seeds germination and seedling growth of Radix isatidis.