大黄鱼鱼卵磷脂对小鼠脂质代谢的调节作用

Effect of Large Yellow Croaker Roe Phospholipid on Lipid Metabolism in Hyperlipidemic Mice

为研究大黄鱼鱼卵磷脂对高脂血症小鼠降血脂作用的影响,通过建立高脂血症小鼠模型,随机分为模型组、空白组、大豆磷脂组以及大黄鱼鱼卵磷脂低(5 g/kg)、中(15 g/kg)和高剂量组(30 g/kg),连续饲料喂养4周后,测定血清相关指标,并进行肝脏病理观察。结果表明:大黄鱼鱼卵磷脂具有抑制小鼠体重增加的作用。血清指标数据分析显示,与模型组相比,大黄鱼鱼卵磷脂低剂量组可显著降低TC、TG水平及AI_1、AI_2值(p<0.05),对降低LDL-C和提高HDL-C水平差异不显著,而中和高剂量组均能显著降低TC、TG和LDL-C水平以及AI_1、AI_2值,可以显著提高HDL-C水平(p<0.05),表明大黄鱼鱼卵磷脂具有调节高脂血症小鼠脂质代谢的作用;小鼠肝脏病理组织学观察显示,大黄鱼鱼卵磷脂低剂量组对小鼠肝细胞无明显影响,而中和高剂量组可以明显修复小鼠肝细胞变性。结果提示大黄鱼鱼卵磷脂可预防小鼠肝脏的脂肪性病变,有助于降低动脉粥样硬化发生的风险。

The hypolipidemic effect of large yellow croaker roe phospholipids was studied using a mouse model of hyperlipidemia. Mice were randomly divided into six groups： model, blank, soybean phospholipid（negative control）, and low-dose（5 g/kg）, medium-dose（15 g/kg） and high-dose（30 g/kg） large yellow croaker roe phospholipid group. After four weeks of feeding, the pathological changes of liver and phospholipid metabolism indices in the serum were investigated. The results indicated that large yellow croaker roe phospholipids suppressed mouse body weight gain. Compared with model group, medium-dose and high-dose large yellow croaker roe phospholipids significantly reduced levels of total cholesterol（TC）, triglycerides（TG）, and the values of atherogenic indexes 1（AI1） and 2（AI2）（p〈0.05）, but had no marked impact on levels of low-density lipoprotein-cholesterol（LDL-C） and high-density lipoprotein-cholesterol（HDL-C）. The findings suggest that medium- and high-dose large yellow croaker roe phospholipids regulated lipid metabolism in hyperlipidemic mice. Based on the results of pathological histology of mouse liver, low-dose large yellow croaker roe phospholipid did not show significant effect on hepatocytes（p〈0.05）, whereas medium- and high-dose large yellow croaker roe phospholipids showed a significant effect on repairing hepatocellular degeneration. The results indicate that large yellow croaker roe phospholipids can prevent fatty degeneration of mouse liver and help reducing the risk of atherosclerosis.