粪便微生物粗提液通过抑制NF-κB信号的抗溃疡性结肠炎研究

Anti-colitis Effect of Fecal Microbiota Crude Extract via Suppressing the Nuclear Factor-κB Signaling Pathway

本文研究了粪便微生物粗提液对人工诱发溃疡性结肠炎小鼠的治疗作用。将64只健康小鼠随机分为4组,即完全空白组(n=20)、肠炎空白组(n=20)、SASP(柳氮硫胺吡啶)组(n=12)和FMT(粪便微生物移植)组(n=12),处理组小鼠自由饮用3%葡聚糖硫酸钠盐(DSS)溶液7 d,建立溃疡性结肠炎(UC)模型;造模后,SASP组、FMT组同时分别给予柳氮硫胺吡啶片及鼠源粪便微生物提取液干预治疗4周,灌胃频率2 d/次,然后进行为期两周的后续观察。结果显示:与肠炎空白组小鼠相比,粪便微生物粗提液使UC小鼠血清IL-1β、IL-6和TNF-α水平显著降低(p<0.05),并降低NF-κB的蛋白表达,增加IκB的蛋白表达。研究表明:粪便微生物粗提液可通过抑制NF-κB信号通路的活化来发挥抗溃疡性结肠炎功效,具有应用于临床医学的潜力。

The effect of crude extract of fecal microbiota on artificially-induced ulcerative colitis in mice was investigated in this study. A total of 64 healthy mice were randomly divided into four groups： blank（n=20）, enteritis blank（n=20）, sulfasalazine（SASP, n=12）, and fecal microbiota transplantation（FMT, n=12）. Mice assigned to the treated groups were allowed to freely drink 3% dextran sulfate sodium salt（DSS） solution for seven days, to establish the ulcerative colitis（UC） model. Subsequently, mice in the SASP and FMT groups were treated with SASP solution and crude extract of fecal microbiota for four weeks, respectively. Mice were observed for two weeks with a gavage performed once every two days. The results showed that compared with the enteritis blank group, crude extract of fecal microbiota significantly decreased serum interleukin（IL）-6, IL-1β, and tumor necrosis factor（TNF）-α levels（p〈0.05）, reduced nuclear factor（NF）-κB protein expression, and increased the level of IκB protein expression. The results indicate that crude extract of fecal microbiota exerts an anti-colitis effect by suppressing activation of the NF-κB signaling pathway and has potential applications in clinical medicine.