补肾调冲方对卵巢早衰大鼠性激素水平及TNF-α、IFN-γ蛋白表达的影响

Effect of Bushen Tiaochong Prescription on sex hormone level and TNF-α and IFN-γ protein expression in rats with premature ovarian failure

目的观察补肾调冲方对卵巢早衰模型大鼠血清促卵泡刺激素(FSH)、促黄体生成素(LH)、抗缪勒管激素(AMH)水平及卵巢组织肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)蛋白表达的影响。方法 60只SD雌性大鼠随机分为6组:对照组,模型组,补肾调冲方低、中、高剂量(以生药计24、12、6 g/kg)组,结合雌激素片(阳性药)组,每组10只。除对照组外,其余各组均ig雷公藤多苷片造模14 d,造模结束24 h后,给予相应药物治疗36 d。给药结束后,酶联免疫吸附(Elisa)法检测大鼠血清FSH、LH、AMH水平,免疫组化法检测卵巢组织TNF-α、IFN-γ蛋白表达。结果与对照组比较,模型组血清FSH、LH水平、卵巢TNF-α、IFN-γ蛋白表达均显著升高,AMH水平显著降低(P<0.05);与模型组比较,各给药组FSH、LH水平、卵巢TNF-α、IFN-γ蛋白表达均显著降低,AMH水平显著升高(P<0.01)。结论补肾调冲方可提高卵巢早衰大鼠卵巢储备能力,抑制卵泡封闭及黄体细胞凋亡,对卵巢早衰可能有治疗作用。

Objective To observe the effect ofBushen Tiaochong Prescription （BTP） on sex hormone level andTNF-α, IFN-γ protein expression in rats with premature ovarian failure. Methods Totally 60 SD female rats were randomly divided into six groups with 10 rats in each group： control group, model group, BTP low, medium, and high dose （24, 12 and 6 g/kg in crude drugs） groups, and Conjugated Estrogens Tablets （positive drug） group. Rats were ig administered with tripterygium glycosides for 14 d to make model of premature ovarian failure except for control group. Rats were treated with corresponding medicines for 36 d 24 h after that. Then the enzyme linked immunosorbent assay （Elisa） method was used to detect the levels ofAMH, LH, and FSH in serum, and the expression of TNF-α and IFN-γ in ovarian tissue was detected by immunohistochemistry. Results Compared with control group, the levels of serum FSH, LH and ovarian TNF-α ,IFN-γ protein expression of model group was increased significantly, and the level of serum AMH was significantly decreased （P 〈 0.05）; Compared with model group, levels of FSH, LH, TNF-α and IFN-γ of BTP groups and Conjugated Estrogens Tablets group were significantly decreased, AMH level was significantly increased （P 〈 0.01） Conclusion BTP can improve the ovarian reserve, inhibit follicle blocking and apoptosis of corpus lutein cells of the premature ovarian rats, which suggests that BTP may have a therapeutic effect on premature ovarian disease.