养血祛风方对抽动障碍大鼠纹状体神经元超微结构的作用研究

Effect of Blood-nourishing and Wind-expelling Formulae on ultrastructure of striatal neurons in tic disorder rats

目的探讨养血祛风方对抽动障碍(TD)大鼠纹状体神经元超微结构的影响。方法采用ig亚氨基二丙腈(IDPN)制备TD大鼠模型,造模成功后将大鼠随机分为模型组,养血祛风方高、中、低剂量(11.75、23.50、35.25 g/kg)组,硫必利(阳性药)组,同时设立对照组,连续给药8周。于给药第1、4、8周进行刻板运动评分;末次给药24 h后,大鼠开颅取纹状体,多聚甲醛固定后切片、染色,通过透射电镜观察纹状体腹侧部超微结构。结果与模型组比较,低、中、高剂量养血祛风方和硫必利均对大鼠刻板运动评分发挥显著改善作用(P<0.05);养血祛风方高剂量组和硫必利组大鼠纹状体中的神经元细胞、线粒体、轴突、树突及微血管结构与模型组比较,具有显著差异。结论养血祛风方对TD模型大鼠脑细胞有保护和修复作用。

Objective To explore the effect of Blood-nourishing and Wind-expelling Formulae （BWF） on ultrastructure of striatal neurons in tic disorder （TD） rats. Methods TD model was induced by ig injection of iminodipropionitrile （IDPN）, then TD rats were divided into model group; BWF low, medium, and high dose （11.75, 23.50 and 35.25 g/kg） groups; and tiapride （positive drug） group by random and meanwhile establish control group. Then the rats were treated for 8 weeks and the stereotyped movement scores were recorded in the first, fourth and eighth week. Operate rats craniotomy for their striatum 24 h after treatment, and then fix, slice, and stain striatum, observe the ultrastructure by transmission electron microscope. Results Compared with model group, low, medium, and high dose of BWF and tiapride significantly improved stereotyped movement score＇s of rats （P 〈 0.05）. TD rats striatum of high dose BWF and tiapride in neuronal cells, mitochondria, axons, dendrites, and microvasculature were signiftcantly different compared with model group. Conclusion BWF plays a role of protection and repairing in TD rats brain cells, which should be paid great attention in the treatment of TD therapy.