摘要
目的探索注射用益气复脉(冻干,YQFM)对环磷酰胺致免疫低下小鼠的影响。方法 ICR小鼠随机分为5组:对照组,模型组,注射用益气复脉高、中、低剂量(1417.00、708.50、354.25 mg/kg)组,尾iv给药,每天1次,连续6 d,于第2天给药后,ip环磷酰胺100 mg/kg制备小鼠免疫低下模型。尾iv印度墨汁检测小鼠炭廓清功能;检测小鼠脾脏指数;ip 5%鸡红细胞悬液检测血清溶血素水平;采用2,4-二硝基氯苯(DNCB)建立耳廓迟发型超敏反应模型,观察各组小鼠耳肿胀度的变化;动物血液分析仪测定小鼠白细胞数和骨髓有核细胞数变化。结果与对照组比较,高、中、低剂量的YQFM均可显著促进免疫低下小鼠的炭廓清功能,显著增加脾脏指数、骨髓有核细胞数和白细胞数(P<0.05、0.01、0.001);高剂量可显著增加溶血素的生成(P<0.05);中、低剂量显著增强迟发型超敏反应(P<0.05、0.01)。结论 YQFM可显著改善环磷酰胺导致的小鼠免疫低下,有望成为一种新的抗肿瘤辅助用药。
Objective To investigate effect ofYiqi Fumai Injection （freeze drying, YQFM） on immunosuppression induced by cytoxan in mice. Methods ICR mice were divided into five groups： control group, model group, YQFM high, medium, and low dose （1417.00, 708.50, and 354.25 mg/kg） groups, iv relative drug in tail once daily for 6 d. Immunosuppression was induced by ip injection ofcytoxan （100 mg/kg） after the second administration of YQFM. Clearance function of mice was detected through iv India ink in tail, and spleen index was detected. Detection of serum hemolysin level was taken by ip injection of 5% red blood cells. Using 2,4-dinitrochlorobenzene （DNCB） to establish the delayed-type hypersensitivity model, the changes of ear swelling degree were observed. Changes in the number of leucocyte and bone marrow nucleated cells in mice were determined by animal blood analyzer. Results Compared with control group, YQFM of high, medium, and low doses could significantly promote the immunosuppressive mice＇s carbon clearance function, and significantly increased the spleen index and the number of leucocyte and bone marrow nucleated cells （P 〈 0.05, 0.01, and 0.001）; high dose YQFM could significantly increase the production of hemolysin （P 〈 0.05）; and low dose significantly enhance delayed-type hypersensitivity （P 〈 0.05 and 0.01）. Conclusion YQFM can significantly improve theimmunosuppression of mice caused by cyclophosphamide, and is expected to become an antineoplastic assistant drug.
出处
《药物评价研究》
CAS
2016年第6期962-965,共4页
Drug Evaluation Research
基金
天津市科技小巨人领军企业培育重大项目(14ZXLJSY00240)
