生地当归药队煎剂的表面增强拉曼散射光谱

Surfaced-Enhanced Raman Scattering Spectroscopic Study on Sheng-Di-Dang-Gui Decoction

为揭示单味煎剂与方剂间的关系,分别测试分析了生地当归药队、单味药生地和单味药当归煎剂的表面增强拉曼散射(surface-enhanced Raman scattering,SERS)光谱,并对其进行谱峰归属。本文主要针对存在于三种煎剂中的17个拉曼信号(538,622,732,761,835,876,959,1 145,1 245,1 276,1 326,1 402,1 456,1 470,1 518,1 546和1 605cm^(-1))进行讨论。生地当归药队煎剂SERS光谱在538,732,761,835,876,959,1 145,1 245,1 276,1 326,1 402,1 456,1 470,1 518和1 605cm^(-1)处,出现15个明显的拉曼信号;生地煎剂SERS光谱在538,761,835,876,959,1 145,1 245,1 276,1 326,1 402,1 470,1 518和1 546cm^(-1)处,出现13个明显的拉曼信号;当归煎剂SERS光谱在538,622,732,761,835,876,959,1 245,1 326和1 402cm^(-1)处,出现10个明显的拉曼信号。生地当归药队煎剂SERS光谱保留了和未观察到生地和当归单味煎剂的某些拉曼峰,且产生了生地和当归单味煎剂中所没有的拉曼信号(1 456和1 605cm^(-1)),即产生新药物成分。生地当归药队煎剂所包含的药物成分并非是生地和当归单味药物煎剂所含药物成分的简单相加。结果表明,SERS光谱可能为方剂研究提供一种高灵敏度、快速准确和操作简单的检测方法。

To reveal the relationship between the single decoction and prescription,the surface-enhanced Raman scattering（SERS）spectroscopy of Sheng-Di-Dang-Gui decoction（SDDGD）,Sheng-Di decoction（SDD）and Dang-Gui decoction（DGD）were tested and analyzed.Mainly seventeen Raman signals（538,622,732,761,835,876,959,1 145,1 245,1 276,1 326,1 402,1 456,1 470,1 518,1 546 and 1 605cm^（-1））in three decoctions were discussed.The characteristic Raman bands of three decoctions were tentatively assigned.Fifteen obvious Raman bands（538,732,761,835,876,959,1 145,1 245,1 276,1 326,1 402,1 456,1 470,1 518 and 1 605cm^（-1））were observed in the SERS spectroscopy of SDDGD,thirteen obvious Raman bands（538,761,835,876,959,1 145,1 245,1 276,1 326,1 402,1 470,1 518 and 1 546cm^（-1））were observed in the SERS spectroscopy of SDD,ten obvious Raman bands（538,622,732,761,835,876,959,1 245,1 326 and 1 402cm^（-1））were observed in the SERS spectroscopy of DGD.Some Raman bands in SERS spectra of SDD and DGD were retained in the SDDGD,however some Raman bands in two kinds of decoctions never appeared in the SDDGD.And new Raman bands（1 456 and 1 605cm^（-1））were generated in the SDDGD,resulted in the fact that new chemical compositions were created.Medical ingredients in the SDDGD were not the simple addition of SDD and DGD.The results showed that the SERS spectroscopy might provide a new kind of novel,effective and simple detecting method for the prescription research.