摘要
目的探讨RNA干扰EMS1基因表达对人胃癌MGC803细胞增殖和迁移的影响。方法为检测下调EMS1基因表达后MGC803细胞功能的变化,运用平皿集落形成实验检测其增殖能力;流式细胞仪分析细胞周期和凋亡;Transwell迁移和侵袭实验观察细胞迁移和侵袭能力的变化。结果下调MGC803细胞中EMS1基因表达后,MGC803细胞增殖、迁移和侵袭能力受到抑制,与对照组相比差异有显著性(P<0.05),而对细胞周期和凋亡无明显影响。结论 RNA干扰EMS1基因可抑制胃癌MGC803细胞的增殖和迁移侵袭能力。
Purpose To explore the effect of EMSI-siRNA on the growth, invasion and migration of human gastric cancer cell line MGC803. Methods It used the Colony formation assay to determine the abilities of proliferation, and flow cytometry analysis to asses cell cycle distribution and apoptosis, transwell invasion and migration experiment to determine the ability of cell invasion and migration after knockdown the expression of EMS1 in MGC803. Results These results suggested that EMS1 gene down-regulated have no affect on cell cycle and cell apoptosis, but the ability of colony formation depressed and migration lowerd obviously （P 〈 0. 05）. Conclusion The results shows EMS1 gene is related to proliferation and migration of tumor.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2016年第12期1317-1322,共6页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金(81172576)
湖南省高校创新平台开放基金(14K085)
河北省卫计委课题(ZD20140219)
关键词
RNA干扰
EMS1基因
增殖
迁移
small interference RNA
EMS1 gene
proliferation
migration
