大夜关门总黄酮提纯工艺及体内体外抗氧化研究

Purification of total flavonoids and study in vivo and in vitro antioxidant of lespedeza cuneata

目的研究大夜关门中总黄酮的提取工艺及体内体外抗氧化性。方法通过超声波提取法提取大夜关门中黄酮类物质,测定羟基自由基清除能力及H_2O_2-FeSO_4自由基发生系统MDA生成的抑制率。结果大夜关门总黄酮最佳提取工艺为:提取温度为70℃,乙醇体积分数为70%,固液比为1∶20,提取时间为60 min。黄酮提取物可有效地清除羟自由基,且对小白鼠肝匀浆自氧化有明显的抑制作用。结论最佳工艺下黄酮提取率为19.43%,具有明显清除自由基及体内抗氧化功能,其医药保健价值有待进一步开发利用。

Objective To study the extraction process of total flavonoids from the lespedeza cuneata and the antioxidant activity in vivo. Methods To extract the flavonoids in the lespedeza cuneata by ultrasonic extraction,the hydroxyl radical scavenging ability and the inhibition rate of H_2O_2-FeSO_4 free radical generation system MDA were determined. Results The optimum extraction process of total flavonoids in bespedeza cuneata was： the extraction temperature was 70℃,ethanol volume fraction was 70%,the ratio of liquid to liquid was 1： 20,the extraction time was 60 min. Flavonoids extract can effectively remove hydroxyl free radicals,and had obvious inhibition effect on the liver homogenate of mice. Conclusion The extraction rate of flavonoids is 19. 43%,which has obvious scavenging free radicals and antioxidant function in vivo,and the value of medical health care should be further developed and utilized.