安康市2013-2014年手足口病实验室检测结果分析

Laboratory tests of hand-foot-mouth disease in Ankang city from 2013 to 2014

目的分析2013-2014年安康市手足口病的病原学特征,为该病预防提供依据。方法对安康市10个区、县送检的手足口病(HFMD)患者临床标本采用实时荧光定量PCR方法进行检测。结果共检测标本1 113份,其中489份肠道通用型病毒核酸阳性,检出率为43.94%;EV71阳性率为31.90%(156/489),Cox Al6阳性率为23.72%(116/489);其他肠道病毒阳性为44.38%(217/489)。不同性别间阳性检出率差异有统计学意义(χ2=6.62,P<0.05),5岁以下儿童为高发人群。结论 2013年和2014年HFMD主要流行病原均为其他肠道病毒,应加强手足口病病原监测,并对其他肠道病毒的进一步分型检测。

Objective To analyze the etiology characteristics of hand- food- mouth disease（ HFMD） in Ankang city from2013 to 2014 and provide scientific evidence for the prevention and control. Methods The clinic specimen of patients with HFMD who were from 10 districts of An Kang were tested by fluorescent quantitation PCR. Results 1 113 cases were detected and 489 were positive,taking up 43. 94% of the total（ 489 /1 113） of which 156 samples were EV71 positive（ 150 /489）,accounting for 31. 90%,116 samples were Cox A16 positives,accounting for 23. 72%（ 116 /489）,and 217 samples were other types of intestinal virus positive,accounting for 44. 38%（ 217 /489）. There were significant differences in the detection rate of viral nucleic acids among different genders. Children aged under 5 years were mostly affected. Conclusion The main of other type of intestinal virus are detected in the intestinal tract in 2013- 2014. So the detection of pathogenic surveillance should be enhanced and other types of intestinal virus of HFMD should be also successively carried on type testing.