菊芋果聚糖:果聚糖1-果糖基转移酶1-FFT基因的克隆及表达分析

Cloning and Expression Analysis of Fructan: fructan 1-fructosyl-transferase 1-FFT Gene in Jerusalem Artichoke

果聚糖作为一种重要的渗透调节物质,与植物碳素分配和抗逆性的密切相关。为明确果聚糖在植物生长发育过程中的作用,本研究以菊芋为材料,克隆出果聚糖:果聚糖1-果糖基转移酶1-FFT基因,其CDS长2 025 bp,编码641个氨基酸,该蛋白的分子量(Mw)为73.906 k D,蛋白的等电点(p I)为10.01。进化树分析显示菊芋中1-FFT基因与维氏菊的1-FFT基因进化距离最近,具有较高的保守性。荧光定量检测结果显示,1-FFT基因在菊芋的叶、根、花、茎和块茎中均有表达,其中在块茎中表达量最高。本研究为解析果聚糖合成酶的分子调控机理及功能鉴定提供依据。

Fructan as an important osmotic regulation substances and is closely related to the carbon distribution and resistance of plants. To identify the role of fructan in plant growth and development process, we used Jerusalem artichoke clone Fructan:fructan 1-fructosyltransferase 1-FFT gene. The CDS of 1-FFT is 2 025 bp in length which presumably code 641 amino acids; Its MW is 51.29 k D with the isoelectric point 10.01. Phylogenetic analysis showed that Jerusalem artichoke 1-FFT gene was closest with Cichorium intybus L. and Lactuca sativa1-FFT gene. q PCR results shows that:1-FFT gene was expressed in the Jerusalem artichoke leaves, roots, flowers,stems and tubers, among the highest expression in the tubers. This study provides further insight into analyzing the molecular regulation mechanism and function of identification in fructan synthetase.